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Smart Polymer–Streptavidin Conjugates [PDF]

ChemInform, 2004
AbstractFor Abstract see ChemInform Abstract in Full Text.
Zhongli Ding, Allan S. Hoffman, Patrick S. Stayton   +2 more
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Autodisplay of streptavidin

Enzyme and Microbial Technology, 2011
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on
Jo Il Kim, Min Park, Sarah Thömmes, Min Jung Kang, Joachim Jose, Jae Chul Pyun   +5 more
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Streptavidin in Antibody Pretargeting. Comparison of a Recombinant Streptavidin with Two Streptavidin Mutant Proteins and Two Commercially Available Streptavidin Proteins

Bioconjugate Chemistry, 1998
In this investigation, a comparison of wild type recombinant streptavidin (r-SAv) with two genetically engineered mutant r-SAv proteins was undertaken. The investigation also included a comparison of the r-SAv with two streptavidin (SAv) proteins from commercial sources. In vitro characterization of the SAv proteins was conducted by HPLC, SDS-PAGE, IEF,
Patrick S. Stayton, Robert L. Vessella, Donald K. Hamlin, L.A. Klumb, Wilbur Ds, Kent R. Buhler, James E. Stray, Richard To   +7 more
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Protein engineering of streptavidin for in vivo assembly of streptavidin beads

Journal of Biotechnology, 2008
Escherichia coli was engineered to intracellularly manufacture streptavidin beads. Variants of streptavidin (monomeric, core and mature full length streptavidin) were C-terminally fused to PhaC, the polyester granule forming enzyme of Cupriavidus necator. All streptavidin fusion proteins mediated formation of the respective granules in E. coli and were
Peters, Verena, Rehm, Bernd HA
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Smart and biofunctional streptavidin

Biomolecular Engineering, 1999
The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits.
Allan S. Hoffman, Patrick S. Stayton, Zhongli Ding, Tsuyoshi Shimoboji, Todd C. McDevitt, Volga Bulmus, Kjell E. Nelson   +6 more
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Genetically engineered avidins and streptavidins

Cellular and Molecular Life Sciences, 2006
Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept ...
Olli H. Laitinen, Henri R. Nordlund, Markku S. Kulomaa, Vesa P. Hytönen   +3 more
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Production of streptavidin in a synthetic medium

Journal of Immunological Methods, 1988
A simple, inexpensive procedure for producing streptavidin has been described. The biotin-binding protein was produced by growing Streptomyces avidinii in a synthetic liquid culture medium containing L-asparagine as the sole nitrogen source. With this procedure, extraneous proteinaceous substances inherently present in culture media prepared with yeast
John E. Butler, John Cazin, M. Suter
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[8] Isolation and properties of streptavidin

1990
Publisher Summary This chapter describes the isolation and characteristics of an alternative biotin-binding protein produced by the bacterium Streptomyces avidinii . The classic method for the isolation of streptavidin from culture broth of S . avidinii involves ammonium sulfate precipitation, ion-exchange chromatography, and crystallization. All
Edward A. Bayer, Meir Wilchek, Haya Ben-Hur   +2 more
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Strong Adverse Contribution of Conformational Dynamics to Streptavidin-Biotin Binding.

Journal of Physical Chemistry B, 2019
Molecular dynamics plays an important role for the biological function of proteins. For protein ligand interactions, changes of conformational entropy of protein and hydration layer are relevant for the binding process.
M. Sarter, Doreen Niether, B. Koenig, W. Lohstroh, M. Zamponi, N. Jalarvo, S. Wiegand, J. Fitter, A. Stadler   +8 more
semanticscholar   +1 more source

Ink Formulation for Inkjet Printing of Streptavidin and Streptavidin Functionalized Nanoparticles

Journal of Dispersion Science and Technology, 2011
Aqueous inks containing the protein streptavidin (SAv) or nanoparticles carrying surface-attached SAv were formulated for piezoelectric inkjet printing, using glycerol and 2-propanol to adjust their dynamic viscosity and their surface tension by variation of concentration and relative ratio of the two organic components.
Veronika Schönhaar, Günter E. M. Tovar, Achim Weber, Thomas Hirth, Kirsten Borchers   +4 more
openaire   +2 more sources