Results 341 to 350 of about 326,708 (370)

[5] Avidin and streptavidin

1990
Publisher Summary This chapter discusses the fundamental molecular properties of avidin and streptavidin. Carbohydrate-free avidin can be obtained by use of deglycosylating enzymes and it has been shown to be present in significant amounts in some commercial preparations from which it may be separated by use of lectin columns.
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Anti-streptavidin IgG antibody interference in anti-cyclic citrullinated peptide (CCP) IgG antibody assays is a rare but important cause of false-positive anti-CCP results

Clinical Chemistry and Laboratory Medicine, 2018
Background: The detection of anti-cyclic citrullinated peptide (anti-CCP) IgG antibodies in blood is mainly used for the diagnosis of rheumatoid arthritis. Falsely elevated anti-CCP IgG antibodies due to anti-streptavidin IgG antibodies were suspected in
M. Berth, S. Willaert, C. de Ridder
semanticscholar   +1 more source

Streptavidin–biotin binding energetics

Biomolecular Engineering, 1999
The high affinity energetics in the streptavidin-biotin system provide an excellent model system for studying how proteins balance enthalpic and entropic components to generate an impressive overall free energy for ligand binding. We review here concerted site-directed mutagenesis, biophysical, and computational studies of aromatic and hydrogen bonding
V. Chu, Isolde Le Trong, David E. Hyre, L.A. Klumb, Ashutosh Chilkoti, Terry P. Lybrand, Patrick S. Stayton, Julie E. Penzotti, Richard To, S. Freitag, Ronald E. Stenkamp   +10 more
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Apparent Hyperthyroidism Caused by Biotin-Like Interference from IgM Anti-Streptavidin Antibodies.

Thyroid, 2018
BACKGROUND Exclusion of analytical interference is important when there is discrepancy between clinical and laboratory findings. However, interferences on immunoassays are often mistaken as isolated laboratory artefacts.
L. Lam, W. Bagg, Geoff Smith, W. Chiu, M. Middleditch, Julie C. Lim, C. Kyle   +6 more
semanticscholar   +1 more source

Immobilized Streptavidin Gradients as Bioconjugation Platforms

Langmuir, 2012
Surface density gradients of streptavidin (SAV) were created on solid surfaces and demonstrated functionality as a bioconjugation platform. The surface density of immobilized streptavidin steadily increased in one dimension from 0 to 235 ng cm(-2) over a distance of 10 mm.
Hans J. Griesser, Kerrilyn R. Diener, Kerrilyn R. Diener, John D. Hayball, John D. Hayball, John D. Hayball, Krasimir Vasilev, Bryan R. Coad, Robert D. Short   +8 more
openaire   +5 more sources

Avidin and streptavidin in clinical diagnostics

Biomolecular Engineering, 1999
With the introduction of the SA/Bi technology into the clinical diagnostic field ultrasensitive tests with an acceptable turn-around time became feasible. Cytokine ELISAs with lower detection limit in the picogram range became finally available. Prior to the introduction of SA/Bi only elevated levels of target could be detected by short time tests but ...
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Colloidal Gold/Streptavidin Methods

1994
Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin for immunocytochemical procedures ...
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A Streptavidin Mutant Containing a Cysteine Stretch That Facilitates Production of a Variety of Specific Streptavidin Conjugates

Nature Biotechnology, 1993
The ability to produce specific streptavidin conjugates has been considerably enhanced by using a streptavidin mutant containing a cysteine stretch, in which sulfhydryl groups serve as unique conjugation sites. A streptavidin molecule containing five cysteine residues at its C-terminus, referred to as Stv-28, was efficiently expressed in Escherichia ...
Cassandra L. Smith, Cassandra L. Smith, Takeshi Sano, Charles R. Cantor, Charles R. Cantor   +4 more
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[18] Avidin- and streptavidin-containing probes

1990
Publisher Summary The collection of avidin-containing probes represents a major partner of the avidin–biotin system. The high number of free lysines provides a good base for extensive derivatization, and the p I of the resultant avidin derivative is reduced to an acceptable level in relation to nonspecific binding.
Meir Wilchek, Edward A. Bayer
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Facile Characterization of the Immobilization of Streptavidin on Magnetic Submicron Particles with a Fluorescent Probe of Streptavidin

Applied Spectroscopy, 2013
To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl- N′-(1-naphthyl)-ethylenediamine (BNEDA) based on Forster resonance energy transfer.
Jialin Qin, Yi Zhang, Gaobo Long, Fei Liao, Jun Pu, Xiaolan Yang, Lin Liu   +6 more
openaire   +3 more sources