Results 71 to 80 of about 363,839 (268)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A Passive Time Reversal Method with a Metamodel for Underwater Source Localization

Journal of Marine Science and Engineering
A passive time reversal method with a metamodel (PTR-MM) is proposed to improve underwater source localization under ocean conditions. PTR-MM eliminates model mismatch errors by replacing the conventional sound propagation model with a Kriging metamodel.
Jiang Liu, Sheng Li
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

On the stability of an Optimal Coalition Structure

, 2010
The two main questions in coalition games are 1) what coalitions should form and 2) how to distribute the value of each coalition between its members. When a game is not superadditive, other coalition structures (CSs) may be more attractive than the grand coalition. For example, if the agents care about the total payoff generated by the entire society,
Airiau, S., Sen, S.
openaire   +3 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Topology optimization for fluid–structure interaction problems considering heat transfer performance

Theoretical and Applied Mechanics Letters
Effectively controlling the deformation and temperature of heated structures is crucial for achieving high-performance active cooling through fluid flow.
Yuhui Jing, Li An, Sinan Yi, Jing Li, Pai Liu, Yaguang Wang, Xiaopeng Zhang   +6 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Engineered Interpenetrating MXene Networks in Aramid Layered Films for Antioxidant and Broadband Electromagnetic Interference Shielding

Advanced Science
MXene‐based layered films featuring a “brick‐and‐mortar” architecture show significant potential for electromagnetic interference (EMI) shielding applications; however, the insulating polymer “mortar” disrupts the connectivity of the MXene network ...
Hongli Cheng, Gaojie Han, Congqi Liu, Yang Zhou, Yiming Wang, Bing Zhou, Yuezhan Feng, Chuntai Liu, Hao‐Bin Zhang, Changyu Shen   +9 more
doaj   +1 more source