Results 81 to 90 of about 5,411,731 (260)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

The Dynamic Characteristics of the Water Entry of a Lifeboat

Journal of Marine Science and Engineering
The International Convention for the Safety of Life at Sea (SOLAS) stipulates that all ships must be equipped with lifesaving devices. The freefall lifeboat has the advantages of simple operation, fast release speed, and good safety performance, so it is
Wenxin Yi, Hui Liu, Jiahe Zou, Conghong Lu, Wenqing Luo, Bo Zhou   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Modal analysis and structural optimization of integrated bladed disks and centrifugal compressor impellers

Reducing carbon emissions is critical, and Carbon Capture, Storage, and Utilization (CCSU) technologies can play a vital role. However, the energy needed for compression is a major obstacle to the success of these technologies.
Lima, Diego Zilli, Ferreira, Wallace Gusmão, Yanagihara, Jurandir Itizo, Dezan, Daniel Jonas, Salviano, Leandro Oliveira [UNESP], Gasparin, Elóy Esteves [UNESP]   +5 more
core   +1 more source

Aircraft morphing wing design by using partial topology optimization

, 2013
A morphing wing concept has been investigated over the last decade because it can effectively enhance aircraft aerodynamic performance over a wider range of flight conditions through structural flexibility.
Bureerat, S., Sleesongsom, S., Tai, K.
core   +1 more source

Numerical Study of Vortex-Induced Vibration Characteristics of a Long Flexible Marine Riser

Journal of Marine Science and Engineering
In ocean engineering, interactions between ocean currents and risers lead to regular vortex shedding on both sides of the riser, causing structural deformation.
Jiahe Zou, Bo Zhou, Wenxin Yi, Conghong Lu, Hui Liu, Wenqing Luo   +5 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Efficient design of experiments for structural optimization using significance screening

, 2011
When performing structural optimization of large scale engineering problems, the choice of experiment design is important. However, classical experiment designs are developed to deal with undesired but inevitable scatter and are thus not ideal for ...
Dersjö, Tomas,, Tomas Dersjö, Mårten Olsson, Olsson, Mårten,   +3 more
core   +1 more source

Engineered Interpenetrating MXene Networks in Aramid Layered Films for Antioxidant and Broadband Electromagnetic Interference Shielding

Advanced Science
MXene‐based layered films featuring a “brick‐and‐mortar” architecture show significant potential for electromagnetic interference (EMI) shielding applications; however, the insulating polymer “mortar” disrupts the connectivity of the MXene network ...
Hongli Cheng, Gaojie Han, Congqi Liu, Yang Zhou, Yiming Wang, Bing Zhou, Yuezhan Feng, Chuntai Liu, Hao‐Bin Zhang, Changyu Shen   +9 more
doaj   +1 more source

A Passive Time Reversal Method with a Metamodel for Underwater Source Localization

Journal of Marine Science and Engineering
A passive time reversal method with a metamodel (PTR-MM) is proposed to improve underwater source localization under ocean conditions. PTR-MM eliminates model mismatch errors by replacing the conventional sound propagation model with a Kriging metamodel.
Jiang Liu, Sheng Li
doaj   +1 more source