Results 101 to 110 of about 4,838,802 (318)
, 2013 Performance comparisons for eukaryotic protein subcellular location prediction method based on the Euk6181 dataset.
Xiaoqi Zheng (333971) +6 more
core +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
FEBS Open Bio, EarlyView.This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Frontiers in Immunology, 2014 Glycoproteins perform extra- and intracellular functions in innate and adaptive immunity by lectin-based interactions to exposed glyco-determinants. Herein, we document and mechanistically explain the formation of subcellular-specific N-glycosylation ...
Ling Yen Lee +4 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Subcellular location of GATA4 wild type and mutant proteins.
, 2013 GFP (green) represents the over expressed GATA4 protein, and DAPI (blue) represents the location of the nucleus. The subcellular localization of pEGFP-GATA4 wild-type (WT) and mutant-type proteins shows that wild-type GATA4 completely localized to the ...
Erli Wang (405970) +11 more
core +1 more source
Threonine 348 regulates the subcellular localization of PTEN
FEBS Open Bio, EarlyView.Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
On the Subcellular Location of Vitamin D Metabolites in Intestine
Journal of Biological Chemistry, 1970 Abstract Following the intravenous injection of radioactive vitamin D, no radioactivity appeared in intestinal chromatin isolated according to the procedure of Marushige and Bonner, J. Mol. Biol., 15, 160 (1966). Instead the radioactivity of intestinal nuclei remained with a membrane fraction which is separated from chromatin at the initial step in the
T C, Chen, J C, Weber, H F, DeLuca
openaire +2 more sources
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
FEBS Open Bio, EarlyView.Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
A multiresolution approach to automated classification of protein subcellular location images
BMC Bioinformatics, 2007 Background Fluorescence microscopy is widely used to determine the subcellular location of proteins. Efforts to determine location on a proteome-wide basis create a need for automated methods to analyze the resulting images.
Srinivasa Gowri +6 more
doaj +1 more source