Results 91 to 100 of about 10,438,012 (377)

A gatekeeper helix determines the substrate specificity of Sjögren–Larsson Syndrome enzyme fatty aldehyde dehydrogenase

Nature Communications, 2014
Mutations in the gene coding for membrane-bound fatty aldehyde dehydrogenase (FALDH) lead to toxic accumulation of lipid species and development of the Sjögren–Larsson Syndrome (SLS), a rare disorder characterized by skin defects and mental retardation ...
M. Keller, U. Zander, Julian E. Fuchs, C. Kreutz, K. Watschinger, T. Mueller, G. Golderer, K. Liedl, M. Ralser, B. Kräutler, E. Werner, J. Márquez   +11 more
semanticscholar   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Investigation on substrate specificity and catalytic activity of serine protease neuropsin

Biophysics and Physicobiology, 2022
Neuropsin is one of serine proteases mainly found at the hippocampus and the amygdala, where it contributes to the long-term potentiation and memory acquisition by rebuilding of synaptic connections.
Masami Lintuluoto, Mitsumasa Abe, Yota Horioka, Yoshifumi Fukunishi, Hideki Tamura, Juha M. Lintuluoto   +5 more
doaj   +1 more source

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

Substrate Specificity of Cytoplasmic N-Glycosyltransferase*

Journal of Biological Chemistry, 2014
Background: N-Glycosyltransferases represent a novel class of N-glycosylation-catalyzing enzymes. Results: A quantitative activity assay allowed us to determine substrate specificities of N-glycosyltransferase.
A. Naegeli, Gaëlle Michaud, M. Schubert, Chia-wei Lin, C. Lizak, T. Darbre, J. Reymond, M. Aebi   +7 more
semanticscholar   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

Probing Steroidal Substrate Specificity of Cytochrome P450 BM3 Variants

Molecules, 2016
M01A82W, M11A82W and M01A82WS72I are three cytochrome P450 BM3 (CYP102A1) variants. They can catalyze the hydroxylation of testosterone (TES) and norethisterone at different positions, thereby making them promising biocatalysts for steroid hydroxylation.
Xing Liu, Zhi-Biao Wang, Ya-Nan Wang, Jian-Qiang Kong   +3 more
doaj   +1 more source

Production of Glycopeptide Derivatives for Exploring Substrate Specificity of Human OGA Toward Sugar Moiety. [PDF]

, 2018
O-GlcNAcase (OGA) is the only enzyme responsible for removing N-acetyl glucosamine (GlcNAc) attached to serine and threonine residues on proteins. This enzyme plays a key role in O-GlcNAc metabolism.
Chen, Xi, Chen, Yi, Guo, Jianshuang, Li, Jing, Li, Shanshan, Li, Yanhong, Wang, Jiajia, Wang, Peng George, Wen, Liuqing, Zang, Lanlan, Zhang, Jiabin, Zhu, Hailiang   +11 more
core  

Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution [PDF]

, 2003
Aldolases have potential as tools for the synthesis of stereochemically complex carbohydrates. Here, we show that directed evolution can be used to alter the stereochemical course of the reaction catalyzed by tagatose-1,6-bisphosphate aldolase.
A. Berry, A. Nelson, AKANUMA, Berry, Blom, Buchholz, Cherry, Crameri, Fessner, Fong, G. J. Williams, Giver, Hall, Jaeger, Kumamaru, Liebeton, Matsumura, May, Merritt, Nakajima, Plater, QAMAR, Raillard, Rutter, S. Domann, Stemmer, Zgiby, Zhao   +27 more
core   +2 more sources

Cyclic nucleotide signaling as a drug target in retinitis pigmentosa

FEBS Letters, EarlyView.
Disruptions in cGMP and cAMP signaling can contribute to retinal dysfunction and photoreceptor loss in retinitis pigmentosa. This perspective examines the mechanisms and evaluates emerging evidence on targeting these pathways as a potential therapeutic strategy to slow or prevent retinal degeneration.
Katri Vainionpää, Ahmed B. Montaser, Henri Leinonen   +2 more
wiley   +1 more source