Results 51 to 60 of about 54,156 (279)
Development of Versatile Vectors for Heterologous Expression in Bacillus
Microorganisms, 2018 The discovery of new enzymes for industrial application relies on a robust discovery pipeline. Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures.
Øivind Larsen +1 more
doaj +1 more source
"Fluctuograms" reveal the intermittent intra-protein communication in subtilisin Carlsberg and correlate mechanical coupling with co-evolution. [PDF]
PLoS Computational Biology, 2011 The mechanism of intra-protein communication and allosteric coupling is key to understanding the structure-property relationship of protein function. For subtilisin Carlsberg, the Ca²+-binding loop is distal to substrate-binding and active sites, yet the
Jordi Silvestre-Ryan +2 more
doaj +1 more source
Crystal structure of subtilisin DY, a random mutant of subtilisin Carlsberg [PDF]
European Journal of Biochemistry, 1998 The crystal structure of subtilisin DY inhibited by N‐benzyloxycarbonyl‐Ala‐Pro‐Phe‐chloromethyl ketone has been solved by molecular replacement with subtilisin Carlsberg as the starting model. The model has been refined to a crystallographic R factor (= Σ∣∣Fo∣−∣Fc∣∣ / Σ∣Fo∣) of 15.1 % using X‐ray diffraction data to 0.175 nm resolution.
S, Eschenburg +6 more
openaire +2 more sources
Advanced Materials, EarlyView.Helper and ionizable lipids play a crucial role in determining ApoE binding and subsequent liver tropism and LDLR‐mediated uptake. Ionizable lipids primarily govern the LDLR‐independent uptake pathway. This complementary interplay between lipid components ultimately governs LNP delivery performance and therapeutic efficacy in the liver.
Ashish Sarode +16 more
wiley +1 more source
Chemically stabilized subtilisins in peptide synthesis [PDF]
, 2008 We have stabilized alcalaseTM and subtilisin Carlsberg (SC) against heat by chemical modification with ethylene glycol bis-succinimidyl succinate (EGNHS), a procedure not previously reported for subtilisins.
Colleary, Sandra, Ó Fágáin, Ciarán
core
Advanced Materials Technologies, EarlyView.Polymer‐coated carbon nanotubes were engineered as protease‐responsive nanocarriers for protein delivery in intact plants. GFP cargo can be released by cytosolic phytaspase cleavage and subsequently targeted to mitochondria via an N‐terminal sequence, enabling controlled intracellular protein delivery without tissue damage and demonstrating stable ...
Simon Sau Yin Law +3 more
wiley +1 more source
The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases [PDF]
, 2002 The protein SpoIVB plays a key role in signaling in the sigma (K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase ...
Brannigan, J A, Cutting, S M, Hoa, N T
core +2 more sources
Electrical Control of the Transduction Channels’ Gating Force in Mechanosensory Hair Cells
Advanced Science, EarlyView.The inner ear's hair cells rely on mechanosensitive ion channels to convert vibrations of their hair‐bundle into electrical signals. We show that varying the electrical potential (U) across the sensory epithelium modulates a key determinant of mechanosensitivity—the gating force (FG)—by modulating the gating swing (d), ranging from the size of the ...
Achille Joliot +2 more
wiley +1 more source
Engineering subtilisin proteases that specifically degrade active RAS
Communications Biology, 2021 Chen et al. describe a rational design of subtilisin mutants that degrade active RAS by cleaving a conserved sequence in switch 2. They further modified the active site to be dependent on a cofactor to generate high target specificity.
Yingwei Chen +18 more
doaj +1 more source
Advanced Science, EarlyView.ARTP mutagenesis yielded Saccharopolyspora spinosa mutant D184 with improved extracellular nitrogen utilization. An integrated workflow of protease genetic manipulation, multi‐omics, and rational synergy design pinpointed a pepP‐clpP‐htpX synergistic triangular combination.
Duo Jin +9 more
wiley +1 more source