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“Fast peaks” in chromatograms of Sudan dyes
Journal of Chromatography A, 2007The analysis of Sudan dyes has gained considerable attention during last years. In several publications multiple peaks in chromatograms of Sudan III and Sudan IV have been detected. It is demonstrated in this work that if the sample is kept in darkness before analysis, only one chromatographic peak appears for Sudan III or Sudan IV.
Ivo Leito, Koit Herodes
exaly +3 more sources
Comparison of the toxicity of the dyes Sudan II and Sudan IV to catalase
Journal of Biochemical and Molecular Toxicology, 2017AbstractThe mechanisms of the toxicity of Sudan dyes to the key antioxidant enzyme catalase (CAT) were investigated by spectroscopic methods, calorimetry techniques, enzyme activity assay, and molecular docking. Results showed that Sudan dyes bound to CAT through hydrophobic force, which changed the microenvironment of tryptophan and tyrosine residues,
Rutao Liu
exaly +3 more sources
Production of the Polyclonal Antibody against Sudan 3 and Immunoassay of Sudan Dyes in Food Samples
Journal of Agricultural and Food Chemistry, 2012In this study, 4-aminophenylacetic acid was covalently coupled to aniline to synthesize an intermediate hapten and the intermediate hapten was coupled to β-naphthol to synthesize a tentative hapten of Sudan 3. The hapten was coupled to bovine serum albumin as the immunogen to produce the polyclonal antibody. The obtained antibody was highly specific to
Jian Ping Wang
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Production of the monoclonal antibody against Sudan 2 for immunoassay of Sudan dyes in egg
Analytical Biochemistry, 2012Many methods have been reported to determine the residues of Sudan dyes in food samples. Among the reported methods, enzyme-linked immunosorbent assay (ELISA) was a frequently used practical screen tool. In this study, a novel hapten of Sudan 2 was synthesized by coupling 4-amino-3-methylbenzoic acid to β-naphthol, and the monoclonal antibody against ...
Jing, Liu +4 more
exaly +3 more sources
Production of the monoclonal antibody against Sudan 4 for multi-immunoassay of Sudan dyes in egg
Food Control, 2012Abstract Sudan dyes (Sudan 1, 2, 3, 4, Para red and Sudan red G) have been banned as food colorants due to their carcinogenicity. This study first synthesized a hapten of Sudan 4 and produced the monoclonal antibody for immunoassay of these red dyes.
Jian Ping Wang
exaly +2 more sources
Determination of banned sudan dyes in chili powder by capillary electrophoresis
Food Chemistry, 2007Abstract A simple and fast method, based on the use of micellar electrokinetic capillary chromatography in combination with UV detection, was developed for the determination of Sudan dyes (I, II, III and IV). The separation of a mixture of the four standards was achieved using a background electrolyte consisting of 5 mM borate (pH 9.3), 20 mM sodium ...
Maria F Mora, Carlos D Garcia
exaly +2 more sources
Biological effects of the Sudan dyes
Biochemical Pharmacology, 1983The hepatic induction of two cytochrome P1-450-mediated activities [aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (ETR)] was studied following the administration of the azo dyes Sudan I, II, III, and IV. When using Ah-responsive C57BL/6J mice, Sudan dye II proved to be quite potent as an inducer causing almost maximal induction at
Ronald A. Lubet +4 more
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Banned Sudan dyes in spices available at markets in Karachi, Pakistan
Food Additives & Contaminants: Part B, 2022Sudan dyes were investigated in branded and non-branded spices, commonly available in the markets of Karachi, Pakistan. High performance liquid chromatography (HPLC) with a variable wavelength detector (VWD) was applied to determine Sudan dyes I-IV. The non-branded samples had higher concentrations of Sudan dyes than the maximum limits of 0.1 mg/kg ...
Asad Ullah +13 more
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The induction of cytochrome P450 1A1 by Sudan Dyes
Journal of Biochemical and Molecular Toxicology, 2008AbstractAzo dyes form a major class of chemically related compounds that are ubiquitous in foods, paints, printing inks, cosmetics, and also used as biological stains in histological and histopathological laboratories and clinics. Sudan I, sudan III, and sudan IV have been classified as category 3 carcinogens by International Agency for Research on ...
Nahla A G Ahmed, Refat +5 more
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