Results 101 to 110 of about 608,326 (393)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Sulfate-reducing bacteria of the human intestine. II. The role in the diseases development

Біологічні студії, 2012
The modern literature data about the role of microflora in the diseases of the large intestine of man are summarized. Special attention is paid to the sulfate-reducing bacteria role in ulcerative colitis development. The basic ways of hydrogen metabolism
I. V. Kushkevych
doaj   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Uranyl Sulfate Nanotubules Templated by N-phenylglycine

Nanomaterials, 2018
The synthesis, structure, and infrared spectroscopy properties of the new organically templated uranyl sulfate Na(phgH+)7[(UO2)6(SO4)10](H2O)3.5 (1), obtained at room temperature by evaporation from aqueous solution, are reported.
Oleg I. Siidra, Evgeny V. Nazarchuk, Dmitry O. Charkin, Nikita V. Chukanov, Wulf Depmeier, Sergey N. Bocharov, Mikhail I. Sharikov   +6 more
doaj   +1 more source

Recovery of Water and Salt from Hyper-Saline Mine Water using Freeze Crystallization [PDF]

, 2019
The Freezerbacks researched, designed, and economically evaluated a full-scale freeze crystallization process as well as two alternative full-scale processes: 5 stage multiple effect evaporation and reverse osmosis.
Casey, Gabrielle
core   +2 more sources

Structural Disorder, Octahedral Coordination, and 2-Dimensional Ferromagnetism in Anhydrous Alums

, 2008
The crystal structures of the triangular lattice, layered anhydrous alums KCr(SO4)2, RbCr(SO4)2 and KAl(SO4)2 are characterized by X-ray and neutron powder diffraction at temperatures between 1.4 and 773 K.
Bazarov, Bramwell, D.V. West, Fehrmann, Graeber, H.W. Zandbergen, Klevtsova, Kohler, Leclaire, Manoli, Oyetola, Pannetier, Piffard, Q. Huang, R.J. Cava, Serrano-Gonzalez, Serrano-Gonzalez, Shirane, T.M. McQueen, Toby, Vegard   +20 more
core   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Quantification of Dehydroepiandrosterone, 17β-Estradiol, Testosterone, and Their Sulfates in Mouse Tissues by LC-MS/MS. [PDF]

, 2019
We report a high-performance, liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) assay to quantify without derivatizaton dehyroepiandrosterone (DHEA), 17β-estradiol (E2), testosterone (T), and their sulfates in serum and tissues.
Napoli, Joseph L, Yoo, Hong Sik
core  

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Ecological assessment of surface water conditions of the Danube river

Вісник Харківського національного університету імені В.Н. Каразіна: Серія Екологія
Purpose. To provide a comprehensive environmental assessment and forecasting of the condition of surface waters of the Danube River within the Ukrainian section (Reni – Izmail – Vylkove), with a focus on the dynamics of key hydrochemical indicators over ...
L. A. Horoshkovа, O. I. Menshov, Y. D. Korniichuk, S. V. Horoshkov, I. V. Ryshykov   +4 more
doaj   +1 more source