Results 211 to 220 of about 16,065 (253)

A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species

Extremophiles, 2013
A newly isolated single-tailed fusiform virus, Sulfolobus tengchongensis spindle-shaped virus STSV2, from Hamazui, China, is characterised. It contains a double-stranded modified DNA genome of 76,107 bp and is enveloped by a lipid membrane structure. Virions exhibit a single coat protein that forms oligomers when isolated.
Erdmann, Susanne, Chen, Bo, Huang, Xiaoxing, Deng, Ling, Liu, Chao, Shah, Shiraz Ali, Bauer, Sven Le Moine, León Sobrino, Carlos, Wang, Haina, Wei, Yunlin, She, Qunxin, Garrett, Roger Antony, Huang, Li, Lin, Lianbing   +13 more
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Recombinant protein expression in Sulfolobus islandicus

2021
Since its invention, recombinant protein expression has greatly facilitated our understanding of various cellular processes in different biological systems because theoretically this technique renders any gene to be expressed in a mesophilic host like Escherichia coli, thus allowing functional characterizations of proteins of interest.
Xu, Feng, Qunxin, She
openaire   +2 more sources

Carboxylase genes of Sulfolobus metallicus

Archives of Microbiology, 1999
Carbon dioxide limitation of Sulfolobus metallicus resulted in increased cellular concentrations of polypeptides that were predicted to be biotin carboxylase and biotin carboxyl-carrier-protein components of a protein complex. These polypeptides were coeluted from a native polyacrylamide gel and were estimated at 19 and 59 kDa after separation by ...
N P, Burton, T D, Williams, P R, Norris
openaire   +2 more sources

A defined cultivation medium for Sulfolobus acidocaldarius.

Journal of Biotechnology, 2019
The thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius is an important model organism for Archaea and genetic systems are well established. To date, the organism is routinely cultivated on complex media based on protein hydrolysates and no common defined medium is established. In this work we address this lack of a standardized defined medium and
Julian Quehenberger, A. Albersmeier, H. Glatzel, M. Hackl, J. Kalinowski, O. Spadiut   +5 more
semanticscholar   +3 more sources

β-Glycosidase from Sulfolobus solfataricus

2001
Publisher Summary The hydrolysis of glycosidic bonds is crucial for several cell functions, such as energy uptake, cell wall expansion, and turnover of signaling molecules. Because the diversity of saccharides, there is a wide variety of glycosyl-hydrolases that have been classified in more than 74 families based on amino acid sequence similarities ...
Moracci M, Ciaramella M, Rossi M
openaire   +5 more sources

Purification and characterization of NADH oxidase from the archaea Sulfolobus acidocaldarius and Sulfolobus solfataricus

Biotechnology and Applied Biochemistry, 1996
The enzyme NADH oxidase (EC 1.6.99.3) has been isolated from the two thermoacidophilic archaea Sulfolobus acidocaldarius and Sulfolobus solfataricus and characterized. In both organisms the enzyme oxidizes specifically beta‐NADH in the presence of molecular oxygen and requires the presence of a flavin cofactor, showing a high specificity for FAD.
MASULLO, Mariorosario, RAIMO G, DELLO RUSSO A, BOCCHINI V, BANNISTER JV   +4 more
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Carboxylesterase from Sulfolobus solfataricus P1

2001
Publisher Summary To date, relatively few investigations regarding the purification and characterization of thermostable (T opt > 60 °) esterases have been conducted. Thus far, esterases from Bacillus acidocaldarius, Pyrococcus furiosus , Bacillus stearothermophilus , Sulfolobus shibatae , Thermoanaerobacterium sp, Pyrococcus abyssi , and ...
A C, Sehgal, W, Callen, E J, Mathur, J M, Short, R M, Kelly   +4 more
openaire   +2 more sources

Sulfolobus tokodaii sp. nov. (f. Sulfolobus sp. strain 7), a new member of the genus Sulfolobus isolated from Beppu Hot Springs, Japan

Extremophiles, 2002
The taxonomic position of a thermoacidophilic crenarchaeote Sulfolobus sp. strain 7, previously isolated from the Beppu Hot Springs in the geothermal area of Kyushu Island, Japan, was investigated by cloning and sequencing, by phylogenetic analysis of the 16S rRNA gene sequence, by DNA-DNA homology with similar species, and by biochemical ...
Toshiharu, Suzuki, Toshio, Iwasaki, Taketoshi, Uzawa, Kurt, Hara, Naoki, Nemoto, Takahide, Kon, Toshiaki, Ueki, Akihiko, Yamagishi, Tairo, Oshima   +8 more
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2-Propanol degradation by Sulfolobus solfataricus

Biotechnology Letters, 2003
Sulfolobus solfataricus used 2-propanol and 2-propanone (acetone) when grown in static cultures at 78 degrees C with or without glucose at 10 g l-1. The presence of 3.92 g 2-propanol l-1 in both cases inhibited growth. However, acetone accumulation following 2-propanol depletion suggested that 2-propanol was co-metabolized via the acetone metabolic ...
Radianingtyas H, Wright PC
openaire   +3 more sources