Results 71 to 80 of about 2,580 (147)
Agrosystems, Geosciences &Environment, Volume 8, Issue 3, September 2025.Abstract As an ecological unit, the rhizosphere microbiome preserves an enormous and largely unexplored diversity of microbes that play essential roles in plant health and soil functions. Omics technologies encompassing high‐throughput approaches such as metagenomics, metatranscriptomics, and metaproteomics provide helpful tools to unravel the ...
Chinenyenwa Fortune Chukwuneme +1 more
wiley +1 more source
Designing Enzymatic Reactivity with an Expanded Palette
ChemBioChem, Volume 26, Issue 11, June 3, 2025.Innovation in biocatalysis is rapidly increasingly the diversity of catalytic reactivity that can be mediated by enzymes, addressing a key bottleneck for their widespread adoption in industrial chemical synthesis. A key approach to this is building enzymes with unnatural catalytic components that provide an expanded palette with new possibilities for ...
Reuben B. Leveson‐Gower
wiley +1 more source
ChemBioChem, Volume 26, Issue 11, June 3, 2025.An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl +7 more
wiley +1 more source
The Archaeal SoxABCD Complex Is a Proton Pump in Sulfolobus acidocaldarius [PDF]
Journal of Biological Chemistry, 1997 The thermoacidophilic archaeon Sulfolobus acidocaldarius expresses a very unusual quinol oxidase, which contains four heme a redox centers and one copper atom. The enzyme was solubilized with dodecyl maltoside and purified to homogeneity by a combination of hydrophobic interaction and anion exchange chromatography.
M, Gleissner +3 more
openaire +2 more sources
Mass Spectrometry Reviews, Volume 44, Issue 3, Page 213-453, May/June 2025.Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley +1 more source
Discovering Hidden Archaeal and Bacterial Lipid Producers in a Euxinic Marine System
Environmental Microbiology, Volume 27, Issue 3, March 2025.We investigated the bacterial and archaeal membrane lipids in the Black Sea, focusing on isoprenoidal and branched GDGTs, combining high‐resolution accurate mass/mass spectrometry with metagenomics. Our objective was to identify the potential biological producers of these lipids and to begin understanding their role in microbial adaptation.
Dina Castillo Boukhchtaber +6 more
wiley +1 more source
Scientific Reports, 2018 Ketol-acid reductoisomerase (KARI) is a bifunctional enzyme in the second step of branched-chain amino acids biosynthetic pathway. Most KARIs prefer NADPH as a cofactor.
Chin-Yu Chen +6 more
doaj +1 more source
Identification, characterization and classification of prokaryotic nucleoid‐associated proteins
Molecular Microbiology, Volume 123, Issue 3, Page 206-217, March 2025.Nucleoid‐associated proteins (NAPs) structure DNA by either bending, wrapping, bridging, or forming protein filaments on the DNA. We review newly identified NAPs and provide a list of simple biochemical assays to study the DNA‐structuring properties of novel NAPs. Abstract Common throughout life is the need to compact and organize the genome.
Samuel Schwab, Remus T. Dame
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Chromatin and gene regulation in archaea
Molecular Microbiology, Volume 123, Issue 3, Page 218-231, March 2025.Recent data from diverse archaea with different repertoires of nucleoid‐associated proteins show a common genome‐wide chromatin organisation where chromatin maintains access to gene promoters independently of transcription activity. Abstract The chromatinisation of DNA by nucleoid‐associated proteins (NAPs) in archaea ‘formats’ the genome structure in ...
Fabian Blombach, Finn Werner
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ESCRT-III mediated cell division in Sulfolobus acidocaldarius –A reconstitution perspective
Frontiers in Microbiology, 2014 In the framework of Synthetic Biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms.
Tobias eHärtel, Petra eSchwille
doaj +1 more source