The genome of Sulfolobus sp. A20 isolated from a hot spring in Costa Rica was sequenced. This circular genome of the strain is 2,688,317 bp in size and 34.8% in G+C content, and contains 2,591 open reading frames (ORFs). Strain A20 shares ~95.6% identity
Xin Dai +16 more
doaj +1 more source
Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [PDF]
Central to genetic work in any organism are the availability of a range of inducible and constitutive promoters. In this work we studied several promoters for use in the hyperthermophilic archaeon Sulfolobus acidocaldarius. The promoters were tested with
Albers, Sonja-Verena +3 more
core
Comparative genomics in acid mine drainage biofilm communities reveals metabolic and structural differentiation of co-occurring archaea [PDF]
Background Metal sulfide mineral dissolution during bioleaching and acid mine drainage (AMD) formation creates an environment that is inhospitable to most life. Despite dominance by a small number of bacteria, AMD microbial biofilm communities contain a
Banfield, Jillian F. +6 more
core +2 more sources
Two enzymes from the Thermus thermophilus lysine biosynthetic pathway, LysZ and LysY, unexpectedly exhibit substrate promiscuity. They are able to recognize the LysW intermediates utilized by the ArgW‐mediated arginine route, even though the bacterium already possesses the canonical arginine biosynthetic pathway.
Wenyuan Shi +3 more
wiley +1 more source
Metabolism of Pentose Sugars in the Hyperthermophilic Archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius [PDF]
We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes d-glucose and d-galactose to pyruvate and glyceraldehyde via a non-phosphorylative version of the Entner-Doudoroff pathway. At each step, one enzyme is active with both C6 epimers, leading to a metabolically promiscuous pathway.
Charlotte E M, Nunn +6 more
openaire +2 more sources
Crenarchaeal biofilm formation under extreme conditions. [PDF]
BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria.
Andrea Koerdt +4 more
doaj +1 more source
Survival and Biofilm Formation of Pseudomonas Putida Under Glucose Starvation on Model Sand Surfaces
P. putida retains viability in sand columns for an extended period of at least 80 days under carbon‐deprivation P. putida increased protein production on their surfaces, enhancing biofilm integrity and adhesion to sand surfaces compared to fed biofilms.
Somayeh Ramezanian +3 more
wiley +1 more source
Characterization of the ATPase FlaI of the motor complex of the Pyrococcus furiosus archaellum and its interactions between the ATP-binding protein FlaH [PDF]
The archaellum, the rotating motility structure of archaea, is best studied in the crenarchaeon Sulfolobus acidocaldarius. To better understand how assembly and rotation of this structure is driven, two ATP-binding proteins, FlaI and FlaH of the motor ...
Paushali Chaudhury +2 more
doaj +2 more sources
Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan +2 more
wiley +1 more source
Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley +1 more source

