Results 61 to 70 of about 2,656,520 (276)

Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells

eLife, 2019
Planar supported lipid bilayers (PSLB) presenting T cell receptor (TCR) ligands and ICAM-1 induce budding of extracellular microvesicles enriched in functional TCR, defined here as synaptic ectosomes (SE), from helper T cells.
David G Saliba, Pablo F Céspedes-Donoso, Štefan Bálint, Ewoud B Compeer, Kseniya Korobchevskaya, Salvatore Valvo, Viveka Mayya, Audun Kvalvaag, Yanchun Peng, Tao Dong, Maria-Laura Tognoli, Eric O'Neill, Sarah Bonham, Roman Fischer, Benedikt M Kessler, Michael L Dustin   +15 more
doaj   +1 more source

Two-dimensional microelectrophoresis in supported lipid bilayers

Biophysical Journal, 1992
We report the application of supported bilayers for two-dimensional microelectrophoresis. This method allows the lateral separation and accumulation of charged amphiphilic molecular probes in bilayers by application of an electric field parallel to the bilayer surface.
Stelzle, M., Miehlich, R., Sackmann, E.
openaire   +2 more sources

Assembly of RNA nanostructures on supported lipid bilayers [PDF]

Nanoscale, 2015
The assembly of nucleic acid nanostructures with controlled size and shape has large impact in the fields of nanotechnology, nanomedicine and synthetic biology.
Aleksandra P, Dabkowska, Agnes, Michanek, Luc, Jaeger, Michael, Rabe, Arkadiusz, Chworos, Fredrik, Höök, Tommy, Nylander, Emma, Sparr   +7 more
openaire   +2 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Silica xerogel/aerogel-supported lipid bilayers: Consequences of surface corrugation [PDF]

, 2010
The objective of this paper was to review our recent investigations of silica xerogel and aerogel-supported lipid bilayers. These systems provide a format to observe relationships between substrate curvature and supported lipid bilayer formation, lipid ...
Xing, Chenyue, Frank, Curtis W., Nellis, Barbara A., Risbud, Subhash H., Satcher, Joe H., Longo, Marjorie L., Faller, Roland, Goksu, Emel I., Hoopes, Matthew I.   +8 more
core   +1 more source

T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles

Nature Communications, 2022
T cells communicate with antigen-presenting cells (APC) via the signaling crosstalk at the immunological synapse (IS). Here the authors use bead-supported lipid bilayers as synthetic APCs to find that trans-synaptic vesicles produced by T cells in the IS
Pablo F. Céspedes, Ashwin Jainarayanan, Lola Fernández-Messina, Salvatore Valvo, David G. Saliba, Elke Kurz, Audun Kvalvaag, Lina Chen, Charity Ganskow, Huw Colin-York, Marco Fritzsche, Yanchun Peng, Tao Dong, Errin Johnson, Jesús A. Siller-Farfán, Omer Dushek, Erdinc Sezgin, Ben Peacock, Alice Law, Dimitri Aubert, Simon Engledow, Moustafa Attar, Svenja Hester, Roman Fischer, Francisco Sánchez-Madrid, Michael L. Dustin   +25 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Difference in molecular mechanisms governing changes in membrane properties of phospholipid bilayers induced by addition of nonionic and zwitterionic surfactants [PDF]

, 2018
We investigated how nonionic surfactants (1-dodecanol, dodecyl methyl ester, and dodecanoic acid) and zwitterionic surfactants (N,N-dimethyldodecylamine N-oxide, and 1-lauroyl-2-hydroxy-sn-glycero-3-phosphatidylcholine) change the physicochemical ...
Sakiho Kitou, Kitou, Sakiho, Susumu Okazaki, Andoh, Yoshimichi, Yoshimichi Andoh, Okazaki, Susumu   +5 more
core   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source