Results 41 to 50 of about 159,531 (259)

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Geometric Evolution of the Chongce Glacier during 1970–2020, Detected by Multi-Source Satellite Observations

open access: yesRemote Sensing, 2021
Glacier surge, which causes a quick movement of ice mass from high to low elevation, is closely associated to the glacial hazards of debris flows and glacial lake outburst floods.
Yongling Sun   +3 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Pinned Droplet Size on a Superhydrophobic Surface in Shear Flow

open access: yesAerospace, 2020
The recent development of a superhydrophobic surface enhances the droplet shedding under a shear flow. The present study gives insights into the effects of shear flow on a pinned droplet over a superhydrophobic surface.
Mitsugu Hasegawa   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Diagnostic and prognostic modeling of glacier dynamics and the driving factors in the Qilian Mountains, China

open access: yesJournal of Hydrology: Regional Studies
Study region: Qiyi Glacier in the Qilian Mountains, China. Study focus: Glacier flow is one of the important processes in the glacier development and evolution.
Sheng Wang   +5 more
doaj   +1 more source

Study on the Influences of the Erosion Wear of Bend Pipe in Testing Surface Process

open access: yesShiyou jixie, 2021
In order to study the influence laws of the nozzles adjustment and the structural parameters of bend pipe on the erosion wear of bend pipe in the operation of offshore surface process, coupled analysis is carried out on the flow velocity in the bend pipe
Wang Jiangang   +4 more
doaj  

Non-linear thermal convection in a

open access: yesAlexandria Engineering Journal, 2016
Casson fluid flow has many practical applications such as food processing, metallurgy, drilling operations and bio-engineering operations. In this paper, we study Casson fluid flow through a plate with a convective boundary condition at the surface and ...
Sachin Shaw   +2 more
doaj   +1 more source

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