Results 121 to 130 of about 247,498 (307)

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Trace gas (CH4, N2O, H2S) distribution in the water column of inland lakes in Poland

Scientific Data
We present a data set of trace gas (TG; CH4, N2O and H2S) concentrations and associated hydrochemical parameters (temperature, EC, pH, ORP, TOC, DOC, HCO3 -, Cl-, $${{\rm{SO}}}_{4}^{2-}$$ SO 4 2 − , NO3 -, δ13CDIC) in the water columns of Polish inland ...
Michał Woszczyk, Mikołaj Majewski, Carsten J. Schubert, Robert van Geldern   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The role of blanket peat moorland management in the generation and amelioration of discolouration of surface water supplies [PDF]

, 2009
Discolouration of surface waters in upland catchments (with associated costs of water treatment and resources) has increased particularly since the severe droughts in the 1970s and mid 1990s.
O'Brien, H, O'Brien, Helen Elizabeth
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Identification and quantification of point sources of surface water contamination in fruit culture in the Netherlands [PDF]

, 2008
Measurements of pesticide concentrations in surface water by the water boards show that they have decreased less than was expected from model calculations.
Zande, J.C., van de, de Werd, H.A.E., Wenneker, M., Beltman, W.H.J., Werd, H.A.E., de, van de Zande, J.C.   +5 more
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Tracing the origins of metalimnetic methane in a stratified large lake: Insights from high‐resolution mapping and three‐dimensional hydrodynamic modeling

Limnology and Oceanography Letters
Methane (CH4) accumulation in the oxic metalimnion is a common yet poorly resolved feature of deep stratified lakes. In Lake Geneva, lateral transport from the Rhône delta has been suggested but lacks spatial validation.
Santona Khatun, Roberto Grilli, Abolfazl Irani Rahaghi, Tomy Doda, Damien Bouffard, Carsten J. Schubert, Jérôme Chappellaz, Marie‐Elodie Perga   +7 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source