MachinesThe contact performance of high-contact-ratio asymmetrical planetary gears is comprehensively evaluated using multiple indicators. The relationship between the indicators and modification parameters is difficult to accurately describe with a single type ...
Yuansheng Zhou +4 more
doaj +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Fast optimization of microwave filters using surrogate-based optimization methods [PDF]
, 2012 This paper investigates the use of surrogate-based optimization to optimize the behavioral response of broadband microwave filters. The proposed method makes use of an efficient infill criterion (called expected improvement) that sequentially samples the
Tom Dhaene +9 more
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Reinforcement Learning-Based Fed-Batch Optimization with Reaction Surrogate Model
, 2021 In this paper, we implement a framework which combines Reinforcement Learning (RL) based reaction optimization with first principle model and plant historical data of the reaction system.
Benton, Michael G. +19 more
core +1 more source
MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis
FEBS Open Bio, EarlyView.MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss +7 more
wiley +1 more source
RF cavity design exploiting a new derivative-free trust region optimization approach
Journal of Advanced Research, 2015 In this article, a novel derivative-free (DF) surrogate-based trust region optimization approach is proposed. In the proposed approach, quadratic surrogate models are constructed and successively updated.
Abdel-Karim S.O. Hassan +4 more
doaj +1 more source
Surrogate-based optimization design for surface texture of helical pair in helical hydraulic rotary actuator. [PDF]
Sci Rep, 2023 Liu S, Li B, Gan R, Xu Y, Yang G.
europepmc +1 more source
Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription
FEBS Open Bio, EarlyView.In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang +10 more
wiley +1 more source