Results 191 to 200 of about 223,298 (311)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

4E analysis of a two-stage refrigeration system through surrogate models based on response surface methods and hybrid grey wolf optimizer. [PDF]

PLoS One, 2023
Ahmed R, Mahadzir S, Mota-Babiloni A, Al-Amin M, Usmani AY, Ashraf Rana Z, Yassin H, Shaik S, Hussain F.   +8 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Machine learning as a tool to engineer microstructures: Morphological prediction of tannin-based colloids using Bayesian surrogate models. [PDF]

MRS Bull, 2022
Jin SA, Kämäräinen T, Rinke P, Rojas OJ, Todorović M.   +4 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Surrogate-based evolutionary optimization of aerodynamic performance and emitted sound for an impeller fan

Centrifugalfans are widely used in building ventilation and air-conditioning systems butface increasing demands for higher energy efficiency, improved performance, and lower noise levels.
Gavin Tabor (21064196), Hamid Motamedi Zokae (22789499), David Moxey (8285391)   +2 more
core  

Mechanical validation of 3D-printed orthopaedic surrogate models for screw insertion training. [PDF]

Eur J Orthop Surg Traumatol
Thomassen A, Lee B, Granger M, Massey P, Chauvin B, Addevico F, Solitro G.   +6 more
europepmc   +1 more source

Human surrogate models of central sensitization: A critical review and practical guide. [PDF]

Eur J Pain, 2021
Quesada C, Kostenko A, Ho I, Leone C, Nochi Z, Stouffs A, Wittayer M, Caspani O, Brix Finnerup N, Mouraux A, Pickering G, Tracey I, Truini A, Treede RD, Garcia-Larrea L.   +14 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A flexible framework for hyperparameter optimization using homotopy and surrogate models. [PDF]

Sci Rep
Abraham SJ, Maduranga KDG, Kinnison J, Carmichael Z, Hauenstein JD, Scheirer WJ.   +5 more
europepmc   +1 more source