Results 71 to 80 of about 1,456,571 (303)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The grain size distribution function of suspended load in the lower Yellow River

River
The log‐normal distribution function (LNDF) and Weibull cumulative density function (WCDF) represent two prevalent approaches for characterizing sediment grain size distributions.
Zipu Ma
doaj   +1 more source

Determination of the Most Appropriate Statistical Method for the Suspended Load Estimation in the Sistan River [PDF]

پژوهش‌های آبخیزداری, 2017
Sedimentation on the rivers beds causes many difficulties not only for an orderly and sustainable flow of water, but also by altering the rivers' hydraulics, which in certain cases drastically changes the flow direction by opening new channels that ...
Naser Rahimi, Farzad Hassanpour, Salman Sharifazari   +2 more
doaj   +1 more source

FIELD OBSERVATION ON SUSPENDED-LOAD IN THE SURF ZONE

Coastal Engineering Proceedings, 1984
Both the concentration of suspended-load and the current velocity have been measured continuously in the surf zone. In order to measure the concentration of the suspended-load in the surf zone, a optical densitometer has been developed.
Kazumasa Katoh, Norio Tanaka, Isao Irie
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Variability of Suspended Sediment Runoff as an Expression of the Long-Term Dynamics of Loess Gully Development—An Example from the Lublin Upland (Eastern Poland)

Land
In the 20-year period from 2003 to 2022, water runoff and suspended sediment load in a forested gully system with a total length of 7.5 km was recorded.
Jan Rodzik, Grzegorz Janicki, Krzysztof Stępniewski, Waldemar Kociuba   +3 more
doaj   +1 more source