Results 71 to 80 of about 423 (305)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

A high step‐up high step‐down coupled inductor based bidirectional DC–DC converter with low voltage stress on switches

open access: yesIET Power Electronics
This paper proposes, a novel soft‐switching bidirectional direct current to direct current (DC–DC) converter based on switched‐capacitor and coupled‐inductor.
Pouya Abolhassani   +5 more
doaj   +1 more source

New modulation scheme for bidirectional qZS modular multi-level converters

open access: yesThe Journal of Engineering, 2019
This study proposes a dedicated modulation scheme for a bidirectional quasi-Z-source (qZS) modular multi-level converter. The operation principle and a suitable pulse-width modulation method are proposed.
Fatma A. Khera   +2 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Hybrid control approach for fast switching MMCs

open access: yesThe Journal of Engineering, 2019
This study presents a new hybrid control approach for different control and switching frequencies for converters of the modular multi-level converter (MMC) family.
Mathias Schnarrenberger   +2 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Analysis and Implementation of an Interleaved High Step‐Up Converter With Zero‐Voltage‐Switching

open access: yesIET Power Electronics
In this paper, a high step‐up DC‐DC converter is presented. The input current ripple is reduced by adding the interleaving technique on the input side.
Marzieh Mohammadi Jouzdani   +3 more
doaj   +1 more source

Non-isolated resonant link DC–DC converter for use with GaN devices

open access: yesThe Journal of Engineering, 2019
As new wide band-gap (WBG) devices are developed and improved, new topologies and control schemes are required to take advantage of the ultra-fast switching turn on/off speeds that are now available, without the limitations of switching losses and ...
Maria Orr   +2 more
doaj   +1 more source

Zero current switching rezonant switch converters [PDF]

open access: yes, 1997
Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.Fen Bilimleri Enstitüsü, Elektrik-Elektronik Ana Bilim DalıIll ...
Yücel, İbrahim
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