Results 81 to 90 of about 420,285 (299)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Estimation of Markov regime-switching regression models with endogenous switching [PDF]

open access: yes
Following Hamilton (1989), estimation of Markov regime-switching regressions nearly always relies on the assumption that the latent state variable controlling the regime change is exogenous.
Richard Startz   +2 more
core  

Hybrid ultra-HVDC system with LCC and cascaded hybrid MMC

open access: yesThe Journal of Engineering, 2019
Nowadays, the hybrid high-voltage direct current (HVDC) system consisting of line commutated converter (LCC) and modular multilevel converter (MMC) has become the most competitive candidate in multi-infeed power systems.
Ruizhang Yang   +3 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Evaluation of DC-link voltage ripple in five-phase PWM voltage source inverters

open access: yesThe Journal of Engineering, 2019
This paper presents the analysis of the DC-link voltage switching ripple in five-phase PWM voltage source inverters with balanced load. The analysis is particularly important for the design and selection of a DC-link capacitor making it possible to meet ...
M. Vujacic   +4 more
doaj   +1 more source

New zero-voltage-switching constant-frequency PWM DC/DC convertor with low switch stress and wide linearity

open access: yesElectronics Letters, 1990
A new zero-voltage-switching (ZVS) DC/DC convertor which operates on constant frequency and has wide linearity is proposed. ZVS operations are achieved not only for the primary switches but also for the secondary rectifier diodes to reduce the switch stresses and losses.
KIM, ID   +2 more
openaire   +2 more sources

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Indirect estimation of Markov switching models with endogenous switching [PDF]

open access: yes
Markov Switching models have been successfully applied to many economic problems. The most popular version of these models implies that the change in the state is driven by a Markov Chain and that the state is an exogenous discrete unobserved variable ...
Calzolari, Giorgio   +2 more
core  

Effects of crosstalk in WDM optical label switching networks due to wavelength switching of a tunable laser [PDF]

open access: yes, 2006
rosstalk caused by switching events in fast tunable lasers in an optical label switching (OLS) system is investigated for the first time. A wavelength-division-multiplexed OLS system based on subcarrier multiplexed labels is presented which employs a 40 ...
Barry, Liam P.   +6 more
core   +2 more sources

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