Results 41 to 50 of about 13,548 (269)

Transition metal ion FRET uncovers K+ regulation of a neurotransmitter/sodium symporter

Nature Communications, 2016
The neurotransmitter transporter SERT counter transport K+ to transport serotonin. Here the authors show that the activity of the prokaryotic orthologue LeuT is also modulated by this cation, suggesting a general regulatory role for K+on neutrotrasmitter:
Christian B. Billesbølle, Jonas S. Mortensen, Azmat Sohail, Solveig G. Schmidt, Lei Shi, Harald H. Sitte, Ulrik Gether, Claus J. Loland   +7 more
doaj   +1 more source

Evolutionary ancestry and novel functions of the mammalian glucose transporter (GLUT) family

BMC Evolutionary Biology, 2010
Background In general, sugar porters function by proton-coupled symport or facilitative transport modes. Symporters, coupled to electrochemical energy, transport nutrients against a substrate gradient.
Patron Nicola, Wilson-O'Brien Amy L, Rogers Suzanne   +2 more
doaj   +1 more source

Modeling of SGLT1 in Reconstituted Systems Reveals Apparent Ion-Dependencies of Glucose Uptake and Strengthens the Notion of Water-Permeable Apo States

Frontiers in Physiology, 2022
The reconstitution of secondary active transporters into liposomes shed light on their molecular transport mechanism. The latter are either symporters, antiporters or exchangers, which use the energy contained in the electrochemical gradient of ions to ...
Thomas Barta, Walter Sandtner, Johann Wachlmayr, Christof Hannesschlaeger, Andrea Ebert, Armin Speletz, Andreas Horner   +6 more
doaj   +1 more source

A conserved leucine occupies the empty substrate site of LeuT in the Na+-free return state

Nature Communications, 2016
Neurotransmitter:sodium symporters (NSS) perform Na+-dependent amino-acid uptake, but a full structural description of their function is lacking. Here the authors present a structure of the bacterial NSS LeuT in the outward-oriented empty state and show ...
Lina Malinauskaite, Saida Said, Caglanur Sahin, Julie Grouleff, Azadeh Shahsavar, Henriette Bjerregaard, Pernille Noer, Kasper Severinsen, Thomas Boesen, Birgit Schiøtt, Steffen Sinning, Poul Nissen   +11 more
doaj   +1 more source

Allosterically Linked Binding Sites in Serotonin Transporter Revealed by Single Molecule Force Spectroscopy

Frontiers in Molecular Biosciences, 2020
Crystal structures and experiments relying on the tools of molecular pharmacology reported conflicting results on ligand binding sites in neurotransmitter/sodium symporters (NSS). We explored the number and functionality of ligand binding sites of NSS in
Rong Zhu, Walter Sandtner, Joan E. A. Ahiable, Amy Hauck Newman, Michael Freissmuth, Harald H. Sitte, Peter Hinterdorfer   +6 more
doaj   +1 more source

The role of transmembrane segment 5 (TM5) in Na2 release and the conformational transition of neurotransmitter:sodium symporters toward the inward-open state

Journal of Biological Chemistry, 2017
Neurotransmitter:sodium symporters (NSSs) terminate neurotransmission by the reuptake of released neurotransmitters. This active accumulation of substrate against its concentration gradient is driven by the transmembrane Na+ gradient and requires that ...
Sebastian Stolzenberg, Zheng Li, M. Quick, L. Malinauskaite, P. Nissen, H. Weinstein, J. Javitch, Lei Shi   +7 more
semanticscholar   +1 more source

Genome-wide identification of the BASS gene family in four Gossypium species and functional characterization of GhBASSs against salt stress

Scientific Reports, 2021
Bile acid sodium symporter (BASS) family proteins encode a class of sodium/solute symporters. Even though the sodium transporting property of BASSs in mammals was well studied, their sodium transportability and functional roles in plant salt tolerance ...
Thwin Myo, Fang Wei, Honghao Zhang, Jianfeng Hao, Bin Zhang, Zhixian Liu, Gangqiang Cao, Baoming Tian, Gongyao Shi   +8 more
doaj   +1 more source

Genetic disruption of lactate/H+ symporters (MCTs) and their subunit CD147/BASIGIN sensitizes glycolytic tumor cells to phenformin.

Cancer Research, 2015
Rapidly growing glycolytic tumors require energy and intracellular pH (pHi) homeostasis through the activity of two major monocarboxylate transporters, MCT1 and the hypoxia-inducible MCT4, in intimate association with the glycoprotein CD147/BASIGIN (BSG).
Ibtissam Marchiq, Renaud Le Floch, D. Roux, M. Simon, J. Pouysségur   +4 more
semanticscholar   +1 more source

A partially-open inward-facing intermediate conformation of LeuT is associated with Na+ release and substrate transport

Nature Communications, 2018
Neurotransmitter:sodium symporters (NSS) modulate the duration and magnitude of signaling via the sodium-coupled reuptake of neurotransmitters. Here the authors describe quantitative single molecule imaging of ligand-induced, functional dynamics of both ...
Daniel S. Terry, Rachel A. Kolster, Matthias Quick, Michael V. LeVine, George Khelashvili, Zhou Zhou, Harel Weinstein, Jonathan A. Javitch, Scott C. Blanchard   +8 more
doaj   +1 more source

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

Biomolecules, 2020
To function as a metabolic hub, plant mitochondria have to exchange a wide variety of metabolic intermediates as well as inorganic ions with the cytosol.
Ian Max Møller, R. Shyama Prasad Rao, Yuexu Jiang, Jay J. Thelen, Dong Xu   +4 more
doaj   +1 more source