Results 21 to 30 of about 7,523 (183)

A partially-open inward-facing intermediate conformation of LeuT is associated with Na+ release and substrate transport

Nature Communications, 2018
Neurotransmitter:sodium symporters (NSS) modulate the duration and magnitude of signaling via the sodium-coupled reuptake of neurotransmitters. Here the authors describe quantitative single molecule imaging of ligand-induced, functional dynamics of both ...
Daniel S. Terry, Rachel A. Kolster, Matthias Quick, Michael V. LeVine, George Khelashvili, Zhou Zhou, Harel Weinstein, Jonathan A. Javitch, Scott C. Blanchard   +8 more
doaj   +1 more source

Anion Selectivity by the Sodium Iodide Symporter [PDF]

Endocrinology, 2003
Abstract The iodide transporter of the thyroid (NIS) has been cloned by the group of Carrasco. The NIS-mediated transport was studied by electrophysiological methods in NIS-expressing Xenopus oocytes. Using this method, the anion selectivity of NIS was different from that previously reported for thyroid cells, whereas perchlorate and ...
Van Sande, Jacqueline, Massart, Claude, Beauwens, Renaud, Schoutens, André, Costagliola, Sabine, Dumont, Jacques Emile, Wolff, J   +6 more
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Allosterically Linked Binding Sites in Serotonin Transporter Revealed by Single Molecule Force Spectroscopy

Frontiers in Molecular Biosciences, 2020
Crystal structures and experiments relying on the tools of molecular pharmacology reported conflicting results on ligand binding sites in neurotransmitter/sodium symporters (NSS). We explored the number and functionality of ligand binding sites of NSS in
Rong Zhu, Walter Sandtner, Joan E. A. Ahiable, Amy Hauck Newman, Michael Freissmuth, Harald H. Sitte, Peter Hinterdorfer   +6 more
doaj   +1 more source

A conserved leucine occupies the empty substrate site of LeuT in the Na+-free return state

Nature Communications, 2016
Neurotransmitter:sodium symporters (NSS) perform Na+-dependent amino-acid uptake, but a full structural description of their function is lacking. Here the authors present a structure of the bacterial NSS LeuT in the outward-oriented empty state and show ...
Lina Malinauskaite, Saida Said, Caglanur Sahin, Julie Grouleff, Azadeh Shahsavar, Henriette Bjerregaard, Pernille Noer, Kasper Severinsen, Thomas Boesen, Birgit Schiøtt, Steffen Sinning, Poul Nissen   +11 more
doaj   +1 more source

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

Biomolecules, 2020
To function as a metabolic hub, plant mitochondria have to exchange a wide variety of metabolic intermediates as well as inorganic ions with the cytosol.
Ian Max Møller, R. Shyama Prasad Rao, Yuexu Jiang, Jay J. Thelen, Dong Xu   +4 more
doaj   +1 more source

Genome-wide identification of the BASS gene family in four Gossypium species and functional characterization of GhBASSs against salt stress

Scientific Reports, 2021
Bile acid sodium symporter (BASS) family proteins encode a class of sodium/solute symporters. Even though the sodium transporting property of BASSs in mammals was well studied, their sodium transportability and functional roles in plant salt tolerance ...
Thwin Myo, Fang Wei, Honghao Zhang, Jianfeng Hao, Bin Zhang, Zhixian Liu, Gangqiang Cao, Baoming Tian, Gongyao Shi   +8 more
doaj   +1 more source

Modeling of SGLT1 in Reconstituted Systems Reveals Apparent Ion-Dependencies of Glucose Uptake and Strengthens the Notion of Water-Permeable Apo States

Frontiers in Physiology, 2022
The reconstitution of secondary active transporters into liposomes shed light on their molecular transport mechanism. The latter are either symporters, antiporters or exchangers, which use the energy contained in the electrochemical gradient of ions to ...
Thomas Barta, Walter Sandtner, Johann Wachlmayr, Christof Hannesschlaeger, Andrea Ebert, Armin Speletz, Andreas Horner   +6 more
doaj   +1 more source

Inhibitor binding mode and allosteric regulation of Na + -glucose symporters

Nature Communications, 2018
Sodium-dependent glucose transporters (SGLTs) transport sugars across the plasma membrane and play important roles in renal sugar reabsorption. Here authors develop structural models of human SGLT1/2 (hSGLT1/2) in complex with inhibitors which helps to ...
Paola Bisignano, Chiara Ghezzi, Hyunil Jo, Nicholas F. Polizzi, Thorsten Althoff, Chakrapani Kalyanaraman, Rosmarie Friemann, Matthew P. Jacobson, Ernest M. Wright, Michael Grabe   +9 more
doaj   +1 more source

Direct assessment of substrate binding to the Neurotransmitter:Sodium Symporter LeuT by solid state NMR

eLife, 2017
The Neurotransmitter:Sodium Symporters (NSSs) represent an important class of proteins mediating sodium-dependent uptake of neurotransmitters from the extracellular space.
Simon Erlendsson, Kamil Gotfryd, Flemming Hofmann Larsen, Jonas Sigurd Mortensen, Michel-Andreas Geiger, Barth-Jan van Rossum, Hartmut Oschkinat, Ulrik Gether, Kaare Teilum, Claus J Loland   +9 more
doaj   +1 more source

Dimerization of the Sodium/Iodide Symporter

Thyroid®, 2019
Background: The ability of thyroid follicular epithelial cells to accumulate iodide via the sodium/iodide symporter (NIS) is exploited to successfully treat most thyroid cancers, although a subset of patients lose functional NIS activity and become unresponsive to radioiodide therapy, with poor clinical outcome.
Thompson, Rebecca J., Fletcher, Alice, Brookes, Katie, Nieto, Hannah, Alshahrani, Mohammed M., Mueller, Jonathan W., Fine, Nicholas H.F., Hodson, David J., Boelaert, Kristien, Read, Martin L., Smith, Vicki E., McCabe, Christopher J.   +11 more
openaire   +2 more sources