A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Structures of rotary ATP synthase from <i>Thermus thermophilus</i> during proton powered ATP synthesis. [PDF]
Sci AdvNakano A +8 more
europepmc +1 more source
HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Iron-catalyzed divergent synthesis of coumarin fused N-heterocycles <i>via</i> 6π-electrocyclization and isoxazole ring-opening. [PDF]
RSC AdvSingh S, Rao MA, Mondal SK, Pal S.
europepmc +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Microwave Synthesis in Zeolite and MOF Membranes. [PDF]
Membranes (Basel)Li L.
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Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
DNA StairLoop: enabling high-fidelity data recovery and robust error correction in DNA-based data storage. [PDF]
Nat CommunYan Z, Qu G, Chen X, Zheng G, Wu H.
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Exam‐level analysis of lecture capture viewing and student exam performance
FEBS Open Bio, EarlyView.This study asks how lecture capture relates to exam performance in biology courses with three noncumulative exams. Mixed‐effects modeling separated between‐students from within‐student effects on exam %. Students who watched more recordings earned higher grades, but viewing spikes above a student's own mean, consistent with cramming, predicted small ...
Kirk Hillsley
wiley +1 more source