Results 111 to 120 of about 2,637,595 (353)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Detection of Enriched T Cell Epitope Specificity in Full T Cell Receptor Sequence Repertoires

open access: yesFrontiers in Immunology, 2019
High-throughput T cell receptor (TCR) sequencing allows the characterization of an individual's TCR repertoire and directly queries their immune state. However, it remains a non-trivial task to couple these sequenced TCRs to their antigenic targets.
Sofie Gielis   +20 more
doaj   +1 more source

Genomic organization of the mouse T-cell receptor β-chain gene family [PDF]

open access: yes, 1987
We have combined three different methods, deletion mapping of T-cell lines, field-inversion gel electrophoresis, and the restriction mapping of a cosmid clone, to construct a physical map of the murine T-cell receptor β-chain gene family.
Barth, Richard K.   +2 more
core  

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

open access: yesFrontiers in Immunology, 2018
While a link between inflammation and the development of neuropsychiatric disorders, including major depressive disorder (MDD) is supported by a growing body of evidence, little is known about the contribution of aberrant adaptive immunity in this ...
Kostas Patas   +18 more
doaj   +1 more source

Binding Site of Human IgG Subclasses and Their Domains for Fc Receptors of Activated Murine T Cells [PDF]

open access: bronze, 1977
Michel Klein   +3 more
openalex   +1 more source

T cell recognition of nonpolymorphic determinants on H-2 class I molecules. [PDF]

open access: yes, 1988
Recognition of polymorphic determinants on class I or class II MHC Ag is required for T lymphocyte responses. Using cell-size artificial membranes (pseudocytes) bearing H-2 class I Ag it is demonstrated that T cells can, in addition, recognize ...
Goldstein, SA, Mescher, MF
core  

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

open access: yesFEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman   +3 more
wiley   +1 more source

Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines

open access: yesClinical and Developmental Immunology, 2004
Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors.
I. Hansenne   +8 more
doaj   +1 more source

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