Results 161 to 170 of about 7,232 (200)

Purification and characterization of recombinant pufferfish (Takifugu rubripes) leptin

General and Comparative Endocrinology, 2008
Synthetic cDNA encoding pufferfish (Takifugu rubripes) leptin (pfLEP) was prepared according to the published sequence. The pfLEP, transformed into Escherichia coli and expressed upon induction with nalidixic acid, was found almost entirely in the insoluble inclusion bodies (IBs).
M, Yacobovitz, G, Solomon, E E, Gusakovsky, B, Levavi-Sivan, A, Gertler   +4 more
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Mitochondrial DNA sequence of the hybrid of Takifugu flavidus (♀) ×  Takifugu rubripes (♂)

Mitochondrial DNA Part A, 2014
The complete mitochondrial genome of the hybrid of Takifugu flavidus (♀) × Takifugu rubripes (♂) was sequenced and characterized. The total length of the mitogenome is 16,443 bp. It contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: origin of light-strand replication (OL) and control region (D-loop).
Hong-Bo, Jiang, Jie, Bao, Ying, Han
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Protein profiling analysis of skeletal muscle of a pufferfish, Takifugu rubripes

Molecular Biology Reports, 2009
Protein profile of the skeletal muscle of Takifugu rubripes, a kind of pufferfish, was carried out with two-dimensional polyacrylamide gel electrophoresis (2-DE). Among the 112 protein spots detected in a silver-stained 2-D polyacrylamide gel, 33 were analyzed by Matrix Assisted Laser Desorption Ionisation tandem time-of-flight mass spectrometry (MALDI
Jian, Lu, Jianzhou, Zheng, Haijun, Liu, Jun, Li, Huayou, Chen, Keping, Chen   +5 more
openaire   +2 more sources

Identification of pepsinogen gene in the genome of stomachless fish, Takifugu rubripes

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2005
Pepsinogen is a precursor of pepsin, a gastric specific protease belonging to the aspartic proteinase family. In teleosts, several species, such as zebrafish and puffer, have independently lost gastric glands. So whether puffer have pepsinogen gene or not is an interesting issue. A search of GSS database for pufferfish, Takifugu rubripes, revealed five
Tadahide, Kurokawa, Susumu, Uji, Tohru, Suzuki   +2 more
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Differences in DNA methylation between slow and fast muscle in Takifugu rubripes

Gene, 2021
Fish skeletal muscle is comprised of fast muscle (FM) and slow muscle (SM), which constitutes 60% of total the body mass. Fish skeletal muscle can affect fish swimming activity, which is important for aquaculture due to its growth-potentiating effects. DNA methylation can influence gene expression level. We previously identified multiple differentially
Haoze, Wang, Jun, Cui, Xuemei, Qiu, Xiuli, Wang   +3 more
openaire   +2 more sources

Collagen of the skin of ocellate puffer fish (Takifugu rubripes)

Food Chemistry, 2002
Abstract Collagens (acid-solubilized and pepsin-solubilized collagens) were prepared from ocellate puffer fish skin and partially characterized. With respect to the pepsin-solubilized collagen, it was a heterotrimer with a chain composition of (α1) 2 α2. The patterns of peptide fragments were different from skin collagens of other species.
Takeshi Nagai, Yoko Araki, Nobutaka Suzuki   +2 more
openaire   +1 more source

Identification of sex markers by cDNA-AFLP in Takifugu rubripes

Aquaculture, 2006
Abstract We searched for sex markers in mature male and female Takifugu rubripes gonads and tailfins by cDNA-amplified fragment length polymorphism (AFLP) performed on pooled cDNA samples. Five sex markers (TDF1-5) were identified, cloned, characterized and confirmed on individual samples of cDNA and genomic DNA, showing good agreement with ...
Jian-Zhou Cui, Xue-Yan Shen, Qing-Li Gong, Guan-Pin Yang, Qian-Qun Gu   +4 more
openaire   +1 more source

Low-salinity tolerance of juvenile fugu Takifugu rubripes

Fisheries Science, 2005
To study the adaptability of juvenile fugu Takifugu rubripes to low-salinity environments, fish were transferred from full-strength seawater (100% SW) to freshwater (FW) and 25, 50, 75 and 100% SW, and checked for mortality over 3 days. No mortality was observed in 25–100% SW, whereas all fish died in FW.
Kyung Mi LEE, Toyoji KANEKO, Katsumi AIDA   +2 more
openaire   +1 more source

Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes

Fish & Shellfish Immunology, 2007
We have cloned cDNAs encoding the alpha and beta chains of CD8 from the tiger pufferfish (fugu), Takifugu rubripes. The cDNA sequences encode a putative leader peptide, extracellular immunoglobulin variable region-like domain, stalk region, transmembrane region, and cytoplasmic tail.
Hiroaki, Suetake, Kyosuke, Araki, Kanako, Akatsu, Tomonori, Somamoto, Johannes Martinus, Dijkstra, Yasutoshi, Yoshiura, Kiyoshi, Kikuchi, Yuzuru, Suzuki   +7 more
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Pharmacokinetics of tetrodotoxin in puffer fish Takifugu rubripes by a single administration technique

Toxicon, 2008
Marine puffer fish accumulates tetrodotoxin (TTX) in the liver and ovary. In this study, we examined the pharmacokinetics of TTX in Takifugu rubripes by a single administration under general anesthesia at 20 degrees C for 300 min. The blood concentration-time profile showed multiple distinct phases after injection into hepatic portal vein.
Takuya, Matsumoto, Yuji, Nagashima, Hiroyuki, Kusuhara, Shoichiro, Ishizaki, Kuniyoshi, Shimakura, Kazuo, Shiomi   +5 more
openaire   +2 more sources