Results 101 to 110 of about 162,388 (137)

Tuning reductase activity in monoterpene indole alkaloid biosynthesis. [PDF]

Proc Natl Acad Sci U S A
Carr SC, Wu S, Gase K, Nakamura Y, Kamileen MO, Kunert M, Heinicke S, Kang M, Grabe V, Caputi L, O'Connor SE.   +10 more
europepmc   +1 more source

Stereoselective deprotonation installs an unusual <i>Z</i>-8,9 double bond during biosynthesis of the diterpene pheromone sobralene.

Chem Commun (Camb)
Da Silva IFP, Ducker C, Pickett JA, Santana AEG, Oldham NJ.   +4 more
europepmc   +1 more source

Molecular dynamics study of taxadiene synthase catalysis [PDF]

Journal of Computational Chemistry, 2018
Molecular dynamics (MD) simulations have been performed to study the dynamic behavior of noncovalent enzyme carbocation complexes involved in the cyclization of geranylgeranyl diphosphate to taxadiene catalyzed by taxadiene synthase (TXS). Taxadiene and the observed four side products originate from the deprotonation of carbocation intermediates.
Walter Thiel, Thomas Bruck, Gui-Juan Cheng   +2 more
exaly   +6 more sources

Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase [PDF]

Chemistry and Biology, 2000
The committed step in the biosynthesis of the anticancer drug taxol in yew (Taxus) species is the cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene. The enzyme taxadiene synthase catalyzes this complex olefin cation cyclization cascade involving the formation of three rings and three stereogenic centers.Recombinant taxadiene synthase ...
Rodney Croteau, Robert M Coates, David C Williams   +2 more
exaly   +5 more sources

QM/MM study of the taxadiene synthase mechanism [PDF]

Journal of Computational Chemistry, 2019
Combined quantum mechanics/molecular mechanics (QM/MM) calculations were used to investigate the reaction mechanism of taxadiene synthase (TXS). TXS catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to taxadiene (T) and four minor cyclic products. All these products originate from the deprotonation of carbocation intermediates.
Walter Thiel, Andres M Escorcia, Jeaphianne P M van Rijn   +2 more
exaly   +5 more sources

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Importation of taxadiene synthase into chloroplast improves taxadiene production in tobacco

Planta, 2021
Importation of taxadiene synthase into chloroplasts is important for the efficient heterologous production of taxadiene. Taxadiene, the first committed precursor to taxol, is synthesized from geranylgeranyl pyrophosphate (GGPP) by action of taxadiene synthase (TS).
Jiang Zhang, Ling Chang, Shengchun Li
exaly   +3 more sources

Facile biosynthesis of taxadiene by a newly constructed Escherichia coli strain fusing enzymes taxadiene synthase and geranylgeranyl pyrophosphate synthase

Process Biochemistry, 2022
Chun-Xiu Li
exaly   +2 more sources

Natural and engineered production of taxadiene with taxadiene synthase

Biotechnology and Bioengineering, 2014
ABSTRACTTaxadiene synthase (TXS) is the rate‐limiting enzyme in the biosynthesis of paclitaxel, an important anticancer compound. TXS catalyzes the conversion of the diterpene precursor geranylgeranyl pyrophosphate (GGPP) into the diterpene taxadiene. Due to the importance of taxadiene in the overall biosynthetic pathway of paclitaxel biosynthesis, the
Sameh, Soliman, Yi, Tang
openaire   +2 more sources

Taxol Production and Taxadiene Synthase Activity inTaxus canadensisCell Suspension Cultures

Archives of Biochemistry and Biophysics, 1997
The cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene represents the first committed, and a slow, step in the complex biosynthetic pathway leading to the anticancer drug Taxol. The cyclization enzyme, taxadiene synthase, has been previously purified from Pacific yew (Taxus brevifolia) stem and characterized, and the corresponding cDNA
Rodney Croteau, Raymond E B Ketchum, D M Gibson   +2 more
exaly   +3 more sources

Overproduction, inEscherichia coli,of Soluble Taxadiene Synthase, a Key Enzyme in the Taxol Biosynthetic Pathway

Protein Expression and Purification, 1998
Taxadiene synthase catalyzes the conversion of the universal precursor of diterpenoids, geranylgeranyl diphosphate, to taxadiene, a key intermediate in Taxol (paclitaxel) biosynthesis. The gene encoding taxadiene synthase was cloned recently. Here we report a method for the heterologous overexpression of cDNA encoding taxadiene synthase in Escherichia ...
Mark R Wildung, Rodney Croteau, A I Scott   +2 more
exaly   +3 more sources