Results 1 to 10 of about 75 (71)
Synthetic and Systems Biotechnology, 2023 Taxadiene is an important precursor for the biosynthesis of highly effective anticancer drug paclitaxel, but its microbial biosynthesis yield is very low. In this study, we employed Yarrowia lipolytica as a microbial host to produce taxadiene.
Qiang Hua, Chun-Xiu Li
exaly +3 more sources
Developing a Yeast Platform Strain for an Enhanced Taxadiene Biosynthesis by CRISPR/Cas9
Metabolites, 2021 Paclitaxel is an important diterpenoid commonly used as an anticancer drug. Although the paclitaxel biosynthetic pathway has been mostly revealed, some steps remain to be elucidated.
Vincent J J Martin +2 more
exaly +3 more sources
Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene [PDF]
BMB Reports, 2012 Paclitaxel is produced by various species of yew trees and hasbeen extensively used to treat tumors. In our research, ataxadiene synthase (TS) gene from Taxus brevifolia was used totransform the roots of cultured ginseng (Panax ginseng C.A.Meyer) to ...
Sang Hee Shim +2 more
exaly +1 more source
Frontiers in Microbiology, 2019 Terpenoids are natural products known for their medicinal and commercial applications. Metabolic engineering of microbial hosts for the production of valuable compounds, such as artemisinin and Taxol, has gained vast interest in the last few decades. The
Hegar Pramastya +2 more
exaly +3 more sources
Frontiers in Bioengineering and Biotechnology, 2023 Taxadiene is an important precursor in taxol biosynthesis pathway, but its biosynthesis in eukaryotic cell factories is limited, which seriously hinders the biosynthesis of taxol.
Chenglong Zhang +7 more
doaj +1 more source
Scientia Pharmaceutica, 2021 Taxadiene synthase (TDS) is the rate-limiting enzyme of Taxol biosynthesis that cyclizes the geranylgeranyl pyrophosphate into taxadiene. Attenuating Taxol productivity by fungi is the main challenge impeding its industrial application; it is possible ...
Ashraf S. A. El-Sayed +7 more
doaj +1 more source
Microbial Cell Factories, 2023 Background Integrated metabolic engineering approaches that combine system and synthetic biology tools enable the efficient design of microbial cell factories for synthesizing high-value products. In this study, we utilized in silico design algorithms on
Koray Malcı +5 more
doaj +1 more source
Biosynthesis of Taxadiene in Saccharomyces cerevisiae : selection of geranylgeranyl diphosphate synthase directed by a computer-aided docking strategy. [PDF]
PLoS ONE, 2014 Identification of efficient key enzymes in biosynthesis pathway and optimization of the fitness between functional modules and chassis are important for improving the production of target compounds.
Ming-Zhu Ding +6 more
doaj +1 more source
Enhanced production of taxadiene in Saccharomyces cerevisiae
Microbial Cell Factories, 2020 Background Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production.
Behnaz Nowrouzi +10 more
doaj +1 more source
Exploiting overflow metabolism in co-cultures for taxadiene biosynthesis [PDF]
RSC SustainabilityThe total biosynthesis of taxadiene is the first step in developing semisynthetic routes to the anticancer drug paclitaxel (Taxol). Existing systems are limited by the metabolic burden of heterologous expression of multiple genes and the cytotoxicity of ...
Avinash Pandreka +6 more
doaj +1 more source