Results 11 to 20 of about 1,120 (154)
Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production [PDF]
Microbial Cell FactoriesBackground Metabolic engineering enables the sustainable and cost-efficient production of complex chemicals. Efficient production of terpenes in Saccharomyces cerevisiae can be achieved by recruiting an intermediate of the mevalonate pathway. The present
Hulya Karaca +7 more
doaj +5 more sources
On the Role of Hydrogen Migrations in the Taxadiene System [PDF]
Angewandte Chemie International Edition, Volume 64, Issue 13, March 24, 2025.Long range proton migrations are known to play a role in the biosynthesis of taxa‐4,11‐diene. This study demonstrates the involvement of long distance hydrogen transfers also in the mass spectrometric fragmentation, and combined approaches through site‐directed mutagenesis and enzymatic conversion of designed substrate analogs give detailed insights ...
Heng Li +2 more
wiley +4 more sources
Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase
ACS Chemical Biology, 2015 The anticancer molecule taxol (Paclitaxel) stands as one of the most medically and economically important natural products. However, despite decades of extensive study, its biosynthesis remains poorly understood. Unpredictable behavior of the first oxygenation enzyme, taxadiene-5α-hydroxylase, which produces a range of undesired products, currently ...
Steven, Edgar +5 more
openaire +3 more sources
Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae [PDF]
Synthetic and Systems BiotechnologyUtilizing synthetic biology techniques to construct recombinant engineered cells for the synthesis of paclitaxel and its key precursors has emerged as an effective method to address the supply–demand imbalance and protect rare plant resources. Taxadiene,
Chenglong Zhang +13 more
doaj +2 more sources
Engineering Salvia miltiorrhiza hairy roots as a scalable platform for high-yield production of paclitaxel and ginsenoside precursors [PDF]
Plant CommunicationsPlant-derived natural products offer a rich source of therapeutic agents. However, sustainable and high-yield production remains a grand challenge. We engineered Salvia miltiorrhiza hairy roots to produce taxadiene, a key precursor for the anticancer ...
Junjie Lin +12 more
doaj +2 more sources
Molecular dynamics study of taxadiene synthase catalysis [PDF]
Journal of Computational Chemistry, 2018 Molecular dynamics (MD) simulations have been performed to study the dynamic behavior of noncovalent enzyme carbocation complexes involved in the cyclization of geranylgeranyl diphosphate to taxadiene catalyzed by taxadiene synthase (TXS). Taxadiene and the observed four side products originate from the deprotonation of carbocation intermediates.
Andrés M. Escorcia +5 more
openaire +5 more sources
Multi‐Omics Analyses Reveal the Red and Far‐Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana [PDF]
Plant, Cell &Environment, Volume 49, Issue 10, Page 7435-7448, October 2026.ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang +6 more
wiley +2 more sources
Designing an oxidase toolbox for site-directed oxidation of taxanes [PDF]
Nature CommunicationsPaclitaxel is one of the most prominent drugs for cancer therapy, and its synthesis has long been pursued by scientific community. Although the biosynthetic pathway has been gradually unraveled, its synthetic efficiency remains severely constrained by ...
Mingyuan Lai +11 more
doaj +2 more sources
Toward Sustainable Paclitaxel Bioproduction: Plant Biology, Biosynthesis and Platform Engineering [PDF]
PlantsPaclitaxel (Taxol), a taxane diterpenoid from Taxus species, is a clinically important microtubule-stabilizing anticancer agent widely used in chemotherapy.
Meng Zhang, Xing Xing, Hongliang Zhu
doaj +2 more sources
Biochemistry, 2018 Taxadiene synthase (TXS) catalyzes the formation of natural product taxa-4(5),11(12)-diene (henceforth taxadiene). Taxadiene is the precursor in the formation of Taxol, which is an important natural anticancer agent. In the current study, we present a detailed mechanistic view of the biosynthesis of taxadiene by TXS using a hybrid quantum mechanics ...
Tamar Ansbacher +2 more
openaire +3 more sources