Results 1 to 10 of about 648 (94)

Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica [PDF]

Synthetic and Systems Biotechnology, 2023
Taxadiene is an important precursor for the biosynthesis of highly effective anticancer drug paclitaxel, but its microbial biosynthesis yield is very low. In this study, we employed Yarrowia lipolytica as a microbial host to produce taxadiene.
Qiang Hua, Chun-Xiu Li
exaly   +4 more sources

Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae [PDF]

Synthetic and Systems Biotechnology
Utilizing synthetic biology techniques to construct recombinant engineered cells for the synthesis of paclitaxel and its key precursors has emerged as an effective method to address the supply–demand imbalance and protect rare plant resources. Taxadiene,
Chenglong Zhang, Jia Wang, Longfei Zhao, Nan Wu, Yi Shi, Xia Li, Changqiang Ke, Jia Liu, Yang Ye, Ying Wang, Bingzhi Li, Wenhai Xiao, Mingdong Yao, Yingjin Yuan   +13 more
doaj   +2 more sources

Engineering Salvia miltiorrhiza hairy roots as a scalable platform for high-yield production of paclitaxel and ginsenoside precursors [PDF]

Plant Communications
Plant-derived natural products offer a rich source of therapeutic agents. However, sustainable and high-yield production remains a grand challenge. We engineered Salvia miltiorrhiza hairy roots to produce taxadiene, a key precursor for the anticancer ...
Junjie Lin, Wei Li, Yang Liu, Furui Yang, Liang Liang, Youran Zeng, Yihong Li, Zheyong Xue, Christopher E. French, Juan Guo, Beimi Cui, Zongsuo Liang, Dongfeng Yang   +12 more
doaj   +2 more sources

Toward Sustainable Paclitaxel Bioproduction: Plant Biology, Biosynthesis and Platform Engineering [PDF]

Plants
Paclitaxel (Taxol), a taxane diterpenoid from Taxus species, is a clinically important microtubule-stabilizing anticancer agent widely used in chemotherapy.
Meng Zhang, Xing Xing, Hongliang Zhu
doaj   +2 more sources

Analysis genome of Pseudotaxus chienii reveals insights into the origin and evolution of taxane biosynthesis [PDF]

Nature Communications
As a sister genus to Taxus, Pseudotaxus holds significant importance for studying the origin and evolution of the taxane biosynthesis pathway. However, the reference genome of Pseudotaxus chienii is yet unavailable.
Mingshuang Wang, Ruoyun Ma, Zijin Fang, Lingxiao Zhang, Yibo Zhang, Mengyin Zheng, Enhui Bai, Wanting Lin, Yanyan Pei, Yue Zang, Huijie Ma, Shangguo Feng, Xiaori Zhan, Chunna Yu, Shuling Wang, Huizhong Wang, Chenliang Yu, Chenjia Shen   +17 more
doaj   +2 more sources

Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production [PDF]

Microbial Cell Factories
Background Metabolic engineering enables the sustainable and cost-efficient production of complex chemicals. Efficient production of terpenes in Saccharomyces cerevisiae can be achieved by recruiting an intermediate of the mevalonate pathway. The present
Hulya Karaca, Murat Kaya, Handan Açelya Kapkac, Serkan Levent, Yusuf Ozkay, Secil Deniz Ozan, Jens Nielsen, Anastasia Krivoruchko   +7 more
doaj   +2 more sources

Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene [PDF]

BMB Reports, 2012
Paclitaxel is produced by various species of yew trees and hasbeen extensively used to treat tumors. In our research, ataxadiene synthase (TS) gene from Taxus brevifolia was used totransform the roots of cultured ginseng (Panax ginseng C.A.Meyer) to ...
Sang Hee Shim, Suk-Yoon Kwon, Kwang-Hyun Baek   +2 more
exaly   +1 more source

Multi-Omics Analyses Reveal the Red and Far-Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana. [PDF]

Plant Cell Environ
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Zhang Y, Wang F, Du R, Li T, Zhou S, Yan J, Li W.   +6 more
europepmc   +2 more sources

TcMYB73, a salicylic acid-responsive R2R3-MYB transcription factor, positively regulates paclitaxel biosynthesis in Taxus chinensis in direct and indirect ways [PDF]

BMC Plant Biology
Background Paclitaxel (Taxol) is an invaluable secondary metabolite extracted from Taxus species, wildly utilized in cancer therapeutics. Salicylic acid (SA), an important phytohormone, substantially elevates paclitaxel accumulation in Taxus cell ...
Yifei Ren, Donglin Liu, Weicheng Zhao, Xinran Wang, Xiaoying Cao, Wen Wan   +5 more
doaj   +2 more sources

Navigating the challenges of engineering composite specialized metabolite pathways in plants. [PDF]

Plant J
SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds are often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels of target metabolites, alternative strategies such as pathway engineering in heterologous systems like ...
Gharat SA, Tamhane VA, Giri AP, Aharoni A.   +3 more
europepmc   +2 more sources