Results 31 to 40 of about 162,388 (137)
Angewandte Chemie International Edition, Volume 61, Issue 43, October 24, 2022., 2022 Two analogues of geranylgeranyl diphosphate with shifted double bond were enzymatically converted with twelve diterpene synthases. The double bond shift causes a change of reactivity that results in the formation of many diterpenes with novel skeletons.
Heng Li, Jeroen S. Dickschat
wiley +1 more source
Exploiting overflow metabolism in co-cultures for taxadiene biosynthesis [PDF]
RSC SustainabilityThe total biosynthesis of taxadiene is the first step in developing semisynthetic routes to the anticancer drug paclitaxel (Taxol). Existing systems are limited by the metabolic burden of heterologous expression of multiple genes and the cytotoxicity of ...
Avinash Pandreka +6 more
doaj +1 more source
, 2023 Taxadiene is an important precursor in taxol biosynthesis pathway, but its biosynthesis in eukaryotic cell factories is limited, which seriously hinders the biosynthesis of taxol.
Tianyu Dong (4764558) +6 more
core +1 more source
A cDNA Clone for Taxadiene Synthase, the Diterpene Cyclase That Catalyzes the Committed Step of Taxol Biosynthesis [PDF]
Journal of Biological Chemistry, 1996 The committed step of taxol (paclitaxel) biosynthesis is catalyzed by taxa-4(5),11(12)-diene synthase, a diterpene cyclase responsible for transforming the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to the parent olefin with a taxane skeleton.
M R, Wildung, R, Croteau
openaire +2 more sources
Cloning of taxadiene synthase gene into Arabidopsis thaliana (ecotype Columbia-0) [PDF]
, 2012 Paclitaxel (Taxol), a complex diterpenoid, produced by yew tree (Taxus sp.) is the most important chemotherapeutic agent that is widely used against a variety of malignancies such as ovarian and breast cancers.
Hejazi, MS +8 more
core +1 more source
, 2016 The mechanism of the taxadiene synthase-catalyzed cyclization of (E,E,E)-geranylgeranyl diphosphate (GGPP, 7) to taxadiene (5) is proposed to proceed through a verticillen-12-yl carbocation intermediate (8) that undergoes an 11 → 7 proton transfer ...
Rodney Croteau (2680519) +3 more
core +2 more sources
Angewandte Chemie, Volume 136, Issue 21, May 21, 2024.Terpene synthases sense their substrates in a binary manner via one of two reactive oxygen atoms. We hypothesize that this alteration in binding, and subsequent chemistry, is due to these enzymes originating from plants or microorganisms. Abstract Terpene synthases (TPSs) catalyze the first step in the formation of terpenoids, which comprise the ...
Renana Schwartz, Shani Zev, Dan T. Major
wiley +2 more sources
Food Frontiers, Volume 7, Issue 4, July 2026.ABSTRACT Grape marc (GM) is a polyphenol‐rich winery byproduct that remains underutilized due to its complex fiber matrix, which limits the bioaccessibility of bound phenolics. While lactic acid fermentation (LAF) can enhance the release of these compounds, the strain‐specific metabolic fate of GM phenolics during digestion and colonic fermentation ...
Ziyao Liu +4 more
wiley +1 more source
FEBS Letters, Volume 600, Issue 11, Page 1621-1637, June 2026.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
, 2022 The green arrows indicate endogenous steps while the blue arrows denote exogenous steps. The green circles highlight the atom transfer route from acetyl-CoA to taxadiene. The abbreviations are: ERG10, acetyl-CoA acetyltransferase; HMGS, HMG-CoA synthase;
Xinfang Song (12400929) +2 more
core +1 more source