Results 51 to 60 of about 162,388 (137)
The second biosynthesis pathway of taxadiene in S. cerevisiae retrieved by PyMiner.
, 2022 Instead of the endogenous HMGR, exogenous enzyme mvaA catalyzes the synthesis of (R)-Mevalonate with the participation of NADH. The green arrows indicate endogenous steps while the blue arrows denote exogenous steps.
Xinfang Song (12400929) +2 more
core +1 more source
Bicistronic expression of nuclear transgenes in Chlamydomonas reinhardtii
The Plant Journal, Volume 118, Issue 5, Page 1400-1412, June 2024.SUMMARY In eukaryotic organisms, proteins are typically translated from monocistronic messenger RNAs containing a single coding sequence (CDS). However, recent long transcript sequencing identified 87 nuclear polycistronic mRNAs in Chlamydomonas reinhardtii natively carrying multiple co‐expressed CDSs.
Nick Jacobebbinghaus +3 more
wiley +1 more source
ChemCatChem, Volume 16, Issue 9, May 8, 2024.A Taxus 2‐O‐benzoyltransferase (mTBT), assisted by CoA, is used to biocatalytically deconstruct older‐generation paclitaxel precursors by removing the 2‐O‐benzoyl group from baccatin III or one of its 10‐O‐acyl analogs. mTBT then appends various non‐natural (m‐substituted)benzoyl groups, transferring them from CoA thioesters to the debenzoylated ...
Aimen Al‐Hilfi +4 more
wiley +1 more source
The fifth biosynthesis pathway of taxadiene in S. cerevisiae identified by PyMiner.
, 2022 Instead of two endogenous reactions catalyzed by ERG8 and ERG19, two exogenous enzymes namely mvaD and ipkA catalyze the synthesis of isopentenyl diphosphate. The green arrows indicate endogenous steps while the blue arrows denote exogenous steps.
Xinfang Song (12400929) +2 more
core +1 more source
Plant Biotechnology Journal, Volume 22, Issue 1, Page 233-247, January 2024.Summary Paclitaxel is one of the most effective anticancer drugs ever developed. Although the most sustainable approach to its production is provided by plant cell cultures, the yield is limited by bottleneck enzymes in the taxane biosynthetic pathway: baccatin‐aminophenylpropanoyl‐13‐O‐transferase (BAPT) and 3′‐N‐debenzoyltaxol N‐benzoyltransferase ...
Edgar Perez‐Matas +4 more
wiley +1 more source
Metabolic engineering of Nicotiana benthamiana for the increased production of taxadiene
, 2014 Key message: We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting.
Hyun Soon Kim +7 more
core +1 more source
The Taxadiene-Forming Carbocation Cascade
, 2016 A complete pathway (structures and energies of intermediates and transition state structures connecting them) from geranylgeranyl diphosphate to taxadiene, obtained using quantum chemical calculations, is described.
Young J. Hong (1346061) +1 more
core +1 more source
Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase
, 2015 The anticancer molecule taxol (Paclitaxel) stands as one of the most medically and economically important natural products. However, despite decades of extensive study, its biosynthesis remains poorly understood.
Kang Zhou (122652) +5 more
core +1 more source
A study into the cobalamin-dependent methionine synthase reactivation mechanism in corynebacterium glutamicum [PDF]
, 2009 PhDMethionine is one of the seven essential amino-acids for humans and is also used in feed supplements, predominantly for poultry and felines. Thus there is commercial interest in producing this nutrient. The final step in the biological synthesis of
Burton-Smith, Raymond Nathaniel
core +4 more sources
Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase [PDF]
, 2011 The structure of ent-copalyl diphosphate synthase (CPS) reveals three α-helical domains (α, β, γ), as also observed in the related diterpene cyclase taxadiene synthase.
Robert M Coates +9 more
core +1 more source