An integrative database and its application for plant synthetic biology research [PDF]
Plant CommunicationsPlant synthetic biology research requires diverse bioparts that facilitate the redesign and construction of new-to-nature biological devices or systems in plants.
Chenfei Tian +7 more
doaj +2 more sources
TcMYB73, a salicylic acid-responsive R2R3-MYB transcription factor, positively regulates paclitaxel biosynthesis in Taxus chinensis in direct and indirect ways [PDF]
BMC Plant BiologyBackground Paclitaxel (Taxol) is an invaluable secondary metabolite extracted from Taxus species, wildly utilized in cancer therapeutics. Salicylic acid (SA), an important phytohormone, substantially elevates paclitaxel accumulation in Taxus cell ...
Yifei Ren +5 more
doaj +2 more sources
Synthetic evolution of Saccharomyces cerevisiae for biomanufacturing: Approaches and applications [PDF]
mLife, Volume 4, Issue 1, Page 1-16, February 2025.Abstract The yeast Saccharomyces cerevisiae is a well‐studied unicellular eukaryote with a significant role in the biomanufacturing of natural products, biofuels, and bulk and value‐added chemicals, as well as the principal model eukaryotic organism utilized for fundamental research.
Zhen Wang +5 more
wiley +2 more sources
Computational analysis of the taxadiene synthase reaction pathway
The taxadiene synthase reaction pathway defines a complex carbocation cascade that offers multiple stable intermediates that, in turn, can lead to multiple relevant products in chemistry. Because of the promiscuity of the terpene chemistry, simple modifications to the natural taxadiene synthase protein can completely modify the potential energy landscape,
Amory, Ross
core +6 more sources
The Impact of Macrocycle Conformation on the Taxadiene-Forming Carbocation Cascade: Insight Gained from Sobralene, a Recently Discovered Verticillene Isomer [PDF]
, 2020 DFT calculations on the carbocation intermediates that connect the biosynthetic pathways leading to the sand fly pheromone sobralene and taxadiene have been made.
Palframan, Matthew J +4 more
core +2 more sources
, 2006 Taxadiene synthase catalyzes the formation of taxadiene from GGPP, the universal building block of diterpenes. The cyclization of GGPP to taxadiene is generally thought to proceed through a series of monocyclic and bicyclic carbocation intermediates, all
Chow, Siew Yin
core +3 more sources
Metabolite profiling identified methylerythritol cyclodiphosphate efflux as a limiting step in microbial isoprenoid production. [PDF]
PLoS ONE, 2012 Isoprenoids are natural products that are all derived from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). These precursors are synthesized either by the mevalonate (MVA) pathway or the 1-Deoxy-D-Xylulose 5-Phosphate (DXP) pathway ...
Kang Zhou +3 more
doaj +1 more source
Cells, 2022 Despite huge progress in biotechnological approaches to paclitaxel production, Taxus spp. in vitro culture productivity still remains a challenge. This could be solved by developing a new strategy engaging mechanisms of the primed defence response joined
Katarzyna Sykłowska-Baranek +4 more
doaj +1 more source
Bioresources and Bioprocessing, 2022 Highlights We report a recombinant Escherichia coli BL21(DE3) strain for de novo production of taxadien-5α-ol, the key precursor of paclitaxel. Through screening of key enzymes and the fermentation condition optimization, the final production of total ...
Qing-Yang Wu +4 more
doaj +1 more source
Profiling of Taxoid Compounds in Plant Cell Cultures of Different Species of Yew (Taxus spp.)
Molecules, 2023 Plant cell cultures of various yew species are a profitable source of taxoids (taxane diterpenoids) with antitumor activity. So far, despite intensive studies, the principles of the formation of different groups of taxoids in cultured in vitro plant ...
Dmitry V. Kochkin +3 more
doaj +1 more source