Results 51 to 60 of about 1,120 (154)

Functional Stability and the Limits of 16S‐Based Inference in Serpentine Biocrusts

Ecological Research, Volume 41, Issue 4, July 2026.
Serpentine geochemistry and moisture regime shape bacterial biocrust communities in the Barberton Greenstone Belt, South Africa. While coarse taxonomic metrics (alpha and beta diversity) showed no significant differences, ASV‐level analyses revealed strong taxonomic shifts linked to soil chemistry and moisture, with numerous serpentine‐associated taxa ...
Danielle Botha, Sandra Barnard, Sarina Claassens, Stefan J. Siebert   +3 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, Volume 600, Issue 11, Page 1621-1637, June 2026.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Synthesis of Amines for Active Pharmaceutical Ingredients Using the Whole‐Cell Factory Saccharomyces Cerevisae

ChemBioChem, Volume 27, Issue 9, 14 May 2026.
This review highlights advances in engineering Saccharomyces cerevisiae for the sustainable biocatalytic production of pharmaceutically relevant amines, amino alcohols, amino acids, and complex alkaloids. It focuses on strategies to increase productivity, including enhanced enzyme expression, cofactor regeneration, precursor channeling, pathway ...
Natalia Kwiatos, Stephen Ossel, Francesco G. Mutti   +2 more
wiley   +1 more source

The Taxadiene-Forming Carbocation Cascade

, 2016
A complete pathway (structures and energies of intermediates and transition state structures connecting them) from geranylgeranyl diphosphate to taxadiene, obtained using quantum chemical calculations, is described.
Young J. Hong (1346061), Dean J. Tantillo (1312941)   +1 more
core   +1 more source

Strategies to recover biological products using solid adsorbents: from polymeric resin beads to the development of novel 3D printed materials [PDF]

, 2023
In the present work, different strategies were used to recover taxadiene from culture media using solid adsorbents. Taxadiene was produced in microbial cultivations using Saccharomyces cerevisiae strain LSR5 as biocatalyst.
Galindo Rodríguez, Giuseppe Rafael
core   +1 more source

Engineering of 10‐Deacetylbaccatin III‐10‐β‐O‐Acetyltransferase From Taxus Species for Efficient Acetylating Non‐Natural Substrates Into Taxol in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 5, Page 3308-3323, May 2026.
ABSTRACT 7‐β‐xylosyl‐10‐deacetyltaxol (XDT) is much more abundant than the anticancer drug Taxol in Taxus species and is usually regarded as the by‐product of Taxol. It could be enzymatically transformed into 10‐deacetyltaxol (DT), and the latter could be further converted into Taxol.
Tian‐Jiao Chen, Jing‐Jing Chen, Xiao‐Yan Sun, Ting Gong, Jin‐Ling Yang, Ping Zhu   +5 more
wiley   +1 more source

In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae

, 2023
In this study, an engineered strain of Saccharomyces cerevisiae was used to produce taxadiene, a precursor in the biosynthetic pathway of the anticancer drug paclitaxel. Taxadiene was recovered in situ with the polymeric adsorbent Diaion © HP-20. Here we
Jorge H. Santoyo-Garcia (15446470), Simone Dimartino (7877030), Giuseppe R. Galindo-Rodriguez (15446467), Leonardo Rios-Solis (12334606)   +3 more
core   +1 more source

Diterpenoid Diversity across Land Plants

Helvetica Chimica Acta, Volume 109, Issue 4, April 2026.
Here we bridge the knowledge of diterpene chemical diversity, biosynthesis, and evolution from nonvascular mosses and liverworts to that known from vascular plants. ABSTRACT The diverse array of diterpenoid natural products stems from the ease of manipulating the promiscuity of diterpene cyclases.
Anita Berg, Chad K. Papenfuhs, Amy E. Fraley   +2 more
wiley   +1 more source

Comprehensive Insights into Natural Bioactive Compounds: From Chemical Diversity and Mechanisms to Biotechnological Innovations and Applications

ChemistryOpen, Volume 15, Issue 4, April 2026.
Natural products derived from plants, animals, fungi, bacteria, and minerals contain diverse bioactive classes such as alkaloids, flavonoids, terpenoids, saponins, tannins, and phenolics. These natural products work through different mechanisms, including ROS inhibition, NF‐κB suppression, and cytokine regulation, and exhibit wide applications across ...
Sajid Ali, Atif Ali Khan Khalil, Muhammad Saeed Akhtar, Adnan Amin, Wajid Zaman   +4 more
wiley   +1 more source

Genome‐scale Metabolic Modeling Guided Escherichia coli Engineering for De Novo Biosynthesis of Chrysanthemic Acid

Advanced Science, Volume 13, Issue 2, 9 January 2026.
Genome‐scale metabolic modeling is preceded to the engineered E. coli cell factory for the de novo biosynthesis of biopesticide chrysanthemic acid. The debranching strategy redirects metabolic flux, enhancing precursor and target compound production.
Jiangpeng Yu, Kelin Cheng, Shenyang Qu, Jie Wang, Xun Wang, Cheng Zhao, Wei Li   +6 more
wiley   +1 more source