Results 71 to 80 of about 1,120 (154)

Taxadiene Synthase-Catalyzed Cyclization of 6-Fluorogeranylgeranyl Diphosphate to 7-Fluoroverticillenes

, 2016
The mechanism of the taxadiene synthase-catalyzed cyclization of (E,E,E)-geranylgeranyl diphosphate (GGPP, 7) to taxadiene (5) is proposed to proceed through a verticillen-12-yl carbocation intermediate (8) that undergoes an 11 → 7 proton transfer ...
Rodney Croteau (2680519), Robert M. Coates (1270191), David C. Williams (2680522), Yinghua Jin (598337)   +3 more
core   +2 more sources

The engineered taxadiene biosynthetic pathway in S. cerevisiae.

, 2014
The engineered taxadiene biosynthetic pathway in S. cerevisiae.
Lu-qing Shang (641243), Ying-jin Yuan (5649157), Lin-feng Li (5660818), Fang Zhai (641242), Zheng Yin (267924), Hui-fang Yan (641241), Ming-zhu Ding (5660815)   +6 more
core   +1 more source

Reanalysis of the biotransformation of 4(20),11(12)-taxadiene derivatives

, 2001
Reanalysis of the biotransformation of 4(20),11(12)-taxadiene derivatives by Absidia coerulea ATCC 10738a unravelled additional unprecedented rearrangement reactions. 5α,7β,9α,10β,13α-pentaacetoxy-4(20),11(12)-taxadiene (1) was converted to 10 new minor
Lolita O Zamir, Di-An Sun, Françoise Sauriol, Orval Mamer   +3 more
core   +1 more source

Cytochrome P450-Mediated Skeleton Rearrangement of Taxadiene in an Engineered Escherichia coli System

In this study, we constructed a taxadiene overproduction platform and identified a cytochrome P450, CYP701A8, that activates the inert C–H bonds in taxadiene to produce three oxidized products (1–3).
Qian Yang (300330), Xiaowei Zhang (6505), Zengyuan Wang (17841682), Xiaoxu Lin (3608474), Hui-Min Xu (8996435), Liao-Bin Dong (1804852), Zhixi Xiao (8461221), Jie Wang (16762), Fang-Ru Li (14164120)   +8 more
core   +2 more sources

Development, characterization and application of 3D printed adsorbents for in situ recovery of taxadiene from microbial cultivations [PDF]

Microbial cell factories are an attractive alternative to produce high-value natural products using sustainable processes. However, product recovery is one of the main challenges to reduce production cost and make these technologies economically ...
Dimartino, Simone, Sarwar, M Sulaiman, Galindo-Rodriguez, Giuseppe Rafael, Rios-Solis, Leonardo   +3 more
core   +1 more source

Probing the Catalytic Promiscuity and Enhancing the Efficiency of CYP725A4 with a Taxadiene Substrate Mimic

The catalytic promiscuity of cytochrome P450 CYP725A4 severely impedes paclitaxel biosynthesis, as it generates taxadien-5α-ol (T-5α-ol) alongside numerous by-products.
Zihan Zhang, Jian-He Xu, Ran Hong, Lili Zhu, Mingyuan Lai, Longhao Huang, Hui-Lei Yu   +6 more
core   +1 more source

AI-Assisted Spatial Metabolic Engineering in Plants: Integrating Flux Design, Spatial Omics, and Synthetic Biology. [PDF]

Metabolites
Chen H, Yang J, Du M.
europepmc   +1 more source

Engineered microorganisms unlock a new era of diterpenoid biomanufacturing: recent advances and future perspectives. [PDF]

Bioresour Bioprocess
Han Y, Luo Y, Wang K, Lin L, Gao J, Sun ML, Ji XJ.   +6 more
europepmc   +1 more source

Way to efficient microbial paclitaxel mass production. [PDF]

Synth Syst Biotechnol, 2023
Li C, Qi Y, Sun Z, Jiang M, Li C.
europepmc   +1 more source

Editing and synthetic engineering of chloroplast genomes. [PDF]

Plant Commun
Fu J, Zhang J.
europepmc   +1 more source