Results 61 to 70 of about 1,120 (154)

Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 1, Page 31-43, January 2026.
Summary In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high‐value peptides, and small molecules produced by heterologous enzymatic pathways.
Maxim D. Harding, Frank Sainsbury
wiley   +1 more source

Accelerating Enzyme Engineering With Artificial Intelligence in Biocatalysis: Challenges and Opportunities

Food Bioengineering, Volume 4, Issue 4, Page 589-611, December 2025.
ABSTRACT Artificial intelligence (AI) has emerged as a paradigm‐shifting force in enzyme engineering, enabling data‐driven prediction of catalytic activity, stability, and substrate specificity. By integrating large‐scale datasets from structured databases (e.g., PDB, BRENDA) and high‐throughput experimentation (e.g., deep mutational scanning ...
Xiafeng Lu, Min Cao, Muqing Ma, Yan Wu, Maohua Qu, Feng Du, Rongtao Ji, Mimi Duan, Leichi Dong, Kai Liu, Xueqi Zhang, Zhibo Luo   +11 more
wiley   +1 more source

Rewiring Saccharomyces cerevisiae metabolism for optimised Taxol® precursors production

Metabolic Engineering Communications
Saccharomyces cerevisiae has been conveniently used to produce Taxol® anticancer drug early precursors. However, the harmful impact of oxidative stress by the first cytochrome P450-reductase enzymes (CYP725A4-POR) of Taxol® pathway has hampered ...
Behnaz Nowrouzi, Pablo Torres-Montero, Eduard J. Kerkhoven, José L. Martínez, Leonardo Rios-Solis   +4 more
doaj   +1 more source

Sustainable Approaches for the Protection and Deprotection of Functional Groups

Chemistry – A European Journal, Volume 31, Issue 40, July 17, 2025.
Shield: Protection and deprotection are key to selective synthesis, but conventional methods can lack sustainability. This review highlights electrochemical and photochemical strategies as greener alternatives, emphasizing their mechanisms, functional group tolerance, and broad synthetic utility.
Ju Ha Jin, Mina An, Minki Jeon, Jaejeong Kim, Sung Min Kang, Isaac Choi   +5 more
wiley   +1 more source

Sorbent‐Based Sampling With Two‐Stage Trapping/Desorption Coupled to Comprehensive Two‐Dimensional Gas Chromatography and Mass Spectrometry for Terpenoids Profiling in Cannabis

Analytical Science Advances, Volume 6, Issue 1, June 2025.
ABSTRACT Cannabis inflorescences represent an important source of many high‐value bioactive specialized metabolites, among which the family of terpenes or terpenoids that are the largest classes of natural products known. Besides their biological activities either alone or synergistic with other terpenoids and/or cannabinoids, they are responsible for ...
Marco De Poli, Tatiana Chenet, Simona Felletti, Damiana Spadafora, Alberto Cavazzini, Flavio A. Franchina   +5 more
wiley   +1 more source

Accessing low-oxidation state taxanes: is taxadiene-4(5)-epoxide on the taxol biosynthetic pathway? [PDF]

, 2016
We have shown for the first time that taxadiene (3) can be epoxidised in a regio- and diastereoselective manner to provide taxadiene-4(5)-epoxide (12) as a single diastereoisomer, and that this epoxide can be rearranged to give taxa-4(20),11(12)-dien-5α ...
Barton, Naomi A., Seymour, Graham B., Lewis, William, Narraidoo, Nathalie, Fray, Rupert George, Hayes, Christopher J., Marsh, Benjamin J.   +6 more
core   +1 more source

Transcriptome Sequencing Reveals Regulatory Mechanisms of Taxol Synthesis in Taxus wallichiana var. Mairei

International Journal of Genomics, 2019
Taxol is one of the most potent and effective anticancer drugs and is originally isolated from Taxus species. To investigate the specific regulatory mechanisms of taxol synthesis in Taxus wallichiana var.
Tao Wang, Yiming Chen, Weibing Zhuang, Fengjiao Zhang, Xiaochun Shu, Zhong Wang, Qing Yang   +6 more
doaj   +1 more source

Navigating the challenges of engineering composite specialized metabolite pathways in plants

The Plant Journal, Volume 121, Issue 6, March 2025.
SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds are often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels of target metabolites, alternative strategies such as pathway engineering in heterologous systems like ...
Sachin A. Gharat, Vaijayanti A. Tamhane, Ashok P. Giri, Asaph Aharoni   +3 more
wiley   +1 more source

Studies on Taxadiene Synthase:  Interception of the Cyclization Cascade at the Isocembrene Stage with GGPP Analogues

, 2016
The cyclization of GGPP to taxadiene, catalyzed by taxadiene synthase, has been suggested to proceed through a series of monocyclic isocembrenyl- and bicyclic verticillyl-carbocationic intermediary stages.
Qiulong Huang (274980), Siew Yin Chow (2662336), A. Ian Scott (2533291), Samik Nanda (2662333), Howard J. Williams (1327818)   +4 more
core   +1 more source

Cloning of taxadiene synthase gene into Arabidopsis thaliana (ecotype Columbia-0) [PDF]

, 2012
Paclitaxel (Taxol), a complex diterpenoid, produced by yew tree (Taxus sp.) is the most important chemotherapeutic agent that is widely used against a variety of malignancies such as ovarian and breast cancers.
Hejazi, MS, Khani, S, Dadpour, MR, Sohani, MM, Omidi, Y, Mahna, N, Atashpaz, S, Nazemieh, H, Barar, J   +8 more
core   +1 more source