Results 151 to 160 of about 26,493 (182)
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Determination of taxol in Taxus chinensis by HPLC method.
Yao xue xue bao = Acta pharmaceutica Sinica, 1992A method for the determination of taxol in Taxus chinensis by HPLC method using betamethasone as internal standard is described. The column employed was packed with 10 microns silica gel YWG 80 (250 x 4 mm), the eluting solvent consisted of CH2Cl2--MeOH (95:5) and the effluent was monitored at 228 nm. The coefficients of variation are less than 2%.
L X, Xu, A R, Liu
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Enhancement of paclitaxel production by temperature shift in suspension culture of Taxus chinensis
Enzyme and Microbial Technology, 2000Effect of temperature shift during culture period on cell growth and paclitaxel was investigated to optimize paclitaxel production in suspension culture of Taxus chinensis. Cell growth showed the optimum at 24 degrees C while paclitaxel synthesis showed the maximum at 29 degrees C. To minimize the inhibitory effect of higher temperature on cell growth,
, Choi +5 more
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Two New Taxoids from the Needles and Stems of Taxus chinensis
HETEROCYCLES, 2005Two new taxoids, named 10-O-benzoyl-15-O-acetyltaxumairol X (1) and 5-O-acetyl-20-O-deacetyl-4,20-p-hydroxylbenzylidenedioxytaxuyunnanine L (2), were isolated from the needles and stems of Taxus chinensis. Their structures were determined on the basis of combined 1D and 2D spectral techniques.
Qin-Shi Zhao +4 more
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Flavonoids of Stems of Taxus chinensis var. mairei
Chemistry of Natural Compounds, 2021Qiang Wei, Shuo Li, Siyue Huang
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[Effect of dissolved oxygen on the suspension culture of Taxus chinensis].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2001The effect of dissolved oxygen controlled level on the taxol production by suspended cell culture of Taxus chinensis was examined in shake flasks and in a 20L aerated and agitated bioreactor with a six-flat-bladed turbine. It was found in shake flasks that oxygen supply exhibited significant influence on the production of taxol.
J, Luo, F, Yu, L, Liu, C D, Wu, X G, Mei
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TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei
Plant Molecular BiologyPaclitaxel is an important natural anticancer drug. Its biosynthesis is very complex and 18 enzymes likely involved have been characterized. However, the regulatory mechanism of these enzyme genes still remains to be elucidated. Here we identified a novel transcription factor in the MYC family of Taxus chinensis TcJAMYC5 function in paclitaxel ...
Shiyu Cai +8 more
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[Chemical constituents of bark of Taxus chinensis var. mairei].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2015To study the chemical constituents in the bark of Taxus chinensis var. mairei collected from southeast of China.Chemical constituents were isolated and purified by column chromatography, Prep-TLC, and preparative HPLC. The structures were identified on the basis of 1D-and 2D-NMR spectral analysis.Twelve taxane diterpenoids were isolated from the bark ...
Jian, Su +5 more
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[Study on HPLC fingerprint of Taxus chinensis var. mairei].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2014To establish the HPLC fingerprint of Taxus chinensis var. mairei collected from different parts during different seasons and provide scientific basis for its comprehensive utilization.Supercritical CO2 extraction was used to extract the effective fraction, HPLC method to establish the fingerprint and similarity evaluation software to analyze the ...
Hui-Qin, Lu +3 more
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Taxol content comparison in different parts of Taxus madia and Taxus chinensis var. mairei by HPLC.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2011To analysis and compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei by High Performance Liquid Chromatography (HPLC).85% EtOH and CH2Cl2 were used for the extraction of taxol. By HPLC, the methodology study and taxol content investigation were performed.The taxol was extracted successfully.
Quan-Feng, Dong +2 more
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