Results 61 to 70 of about 772,937 (315)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

A Suggested Program Based on Social Linguistics Approach to develop Oral Communication skills for Second Language Learners [PDF]

open access: yesمجلة بحوث التعليم والإبتكار
This research aimed to verify the effectiveness of a program based on sociolinguistics in developing oral communication skills among Arabic language learners who speak other languages at the intermediate level.
Asmaa Salah-Eldin Khalifa Hasan   +2 more
doaj   +1 more source

The NCRM quick start guide: Teaching social research methods asynchronously online – guiding principles [PDF]

open access: yes, 2020
The COVID-19 outbreak has prompted a major shift from face-to-face to online teaching, including of research methods. Much of this is asynchronous. Asynchronous elements can include pre-recorded content, discussion boards, assessment activities and ...
Collins, Debbie
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

The Use of Elaboration Theory to Develop Achievement in Grammar among the Preparatory Stage Pupils [PDF]

open access: yesمجلة بحوث التعليم والإبتكار
The current study aimed to reveal the effectiveness of using the Elaboration theory to develop achievement in Arabic grammar among preparatory school students.
Karam Mahmoud Mohamed Madani   +2 more
doaj   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

open access: yesFEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Experiences of teaching a lightweight formal method [PDF]

open access: yes, 2008
This paper reports our experience of using a "lightweight" formal approach, Alloy, and its associated tool support for teaching a core undergraduate module introducing formal methods.
Boyatt, Russell, Sinclair, Jane
core  

YIPFα1A expression is regulated by multilayered molecular mechanisms

open access: yesFEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji   +2 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

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