Results 91 to 100 of about 10,051,843 (305)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Research on Annular Pressure Buildup in Deepwater Oil and Gas Well
Shiyou jixie, 2018 The annulus pressure buildup of deepwater oil and gas wells refers to the phenomenon that the casing annulus fluid expands, owing to the temperature increase, to cause the closed space to generate additional pressure.
Wu Xueting, Wang Jiangshuai, Feng Wei
doaj
Temperature effect on space charge dynamics in XLPE insulation
, 2007 This paper reports on space charge evolution in crosslinked polyethylene (XLPE) planar samples approximately 1.20 mm thick subjected to electric stress level of 30 kVdc/mm under four temperature 25 oC, 50 oC, 70 oC and 90 oC for 24 hours.
Chong, Y L, Chen, G, Ho, Y F F
core
Temperature distribution of 5 cycles.
, 2015 Temperature distribution of 5 cycles.
Liwei Li (804248) +4 more
core +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
, 2012 Temperature effect on: (A) hydrodynamic diameter; (B) PDI; (C) intensity weighted size distribution with increasing temperature; (D) intensity weighted size distribution with decreasing temperature.
Dongxu Zhou (119447) +2 more
core +1 more source
Temperature Distribution in Insulated Temperature-Controlled Container by Numerical Simulation
, 2020 Cold-storage containers are widely used in cold-chain logistics transportation due to their energy saving, environmental protection, and low operating cost.
Yongfeng Cao +7 more
core +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source