Results 111 to 120 of about 219,031 (288)

Assessing Plant Genetic Diversity by Molecular Tools

Diversity, 2009
This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation
Linda Mondini, Arshiya Noorani, Mario A. Pagnotta   +2 more
doaj   +1 more source

Scientism on Steroids: A Review of Freedom Evolves by Daniel Dennett (2003) (review revised 2019) [PDF]

, 2019
``People say again and again that philosophy doesn´t really progress, that we are still occupied with the same philosophical problems as were the Greeks. But the people who say this don´t understand why it has to be so.
Starks, Michael
core  

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Coherent Bayesian analysis of inspiral signals

, 2007
We present in this paper a Bayesian parameter estimation method for the analysis of interferometric gravitational wave observations of an inspiral of binary compact objects using data recorded simultaneously by a network of several interferometers at ...
Acernese F, Acernese F, Andrea Viceré, Arun K G, Arun K G, Baker J G van Meter J R McWilliams S T Centrella J Kelly B J, Beauville F, Beauville F, Blanchet L, Buonanno A Cook G B Pretorius F, Christian Röver, Finn L S, Gelman A, Gianluca M Guidi, Gilks W R, Goldberg D E, Gregory P C, Janka H-T, Jaynes E T, Lück H, Mardia K V, Marion F, Nelson Christensen, Press W H, Renate Meyer, Röver C, Sigg D, Takahashi R, Umstätter R, Umstätter R, van Kerkwijk M H   +30 more
core   +2 more sources

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Loss of heterozygosity (LOH)--implications for human genetic identification.

Folia Histochemica et Cytobiologica, 2009
The aim of this study was assessment of possible effects of loss of heterozygosity on human genetic identification of histolopathogical tissue sections. DNA templates were extracted from tumour tissue specimens excised from oncological patients and from ...
Jerzy Janica, Tomasz Filipowski, Leszek Kozłowski, Renata Zalewska, Marek Rogowski, Małgorzata Skawrońska, Ireneusz Sołtyszewski, Witold Pepiński   +7 more
doaj   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Mutation Screening Using Automated Bidirectional Dideoxy Fingerprinting

BioTechniques, 2000
The need continues to grow for mutation identification in genetic disease in both research and clinical settings. We have developed a rapid nonradioactive bidirectional dideoxy fingerprint mutation screening procedure that is performed using an automated
Yuriy O. Shevchenko, Sherri J. Bale, John G. Compton   +2 more
doaj   +1 more source