Results 91 to 100 of about 121,448 (310)

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Modular Golden Gate Assembly of linear DNA templates for cell-free prototyping [PDF]

Cell-free transcription and translation (TXTL) systems have emerged as a powerful tool for testing genetic regulatory elements and circuits. Cell-free prototyping can dramatically accelerate the design-build-test-learn cycle of new functions in synthetic
Gaizauskaite, A., Inckemann, R., Niederholtmeyer, H. ; https://orcid.org/, Lipińska, K., Gilles, S., Lehr, F., Sahoo, A.   +6 more
core   +1 more source

CRISPR-Enabled Autonomous Transposable Element (CREATE) for RNA-based gene editing and delivery

EMBO Reports
To address a wide range of genetic diseases, genome editing tools that can achieve targeted delivery of large genes without causing double-strand breaks (DSBs) or requiring DNA templates are necessary.
Yuxiao Wang, Ruei-Zeng Lin, Meghan Harris, Bianca Lavayen, Neha Diwanji, Bruce McCreedy, Robert Hofmeister, Daniel Getts   +7 more
doaj   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

Non-Enzymatic Copying of Nucleic Acid Templates [PDF]

, 2016
All known living cells contain a complex set of molecular machinery to support their growth and replication. However, the earliest cells must have been much simpler, consisting of a compartment and a genetic material to allow for Darwinian evolution.
Blain, Jonathan Craig
core  

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes [PDF]

, 2020
CRISPR/Cas9 machinery delivered as ribonucleoprotein (RNP) to the zygote has become a standard tool for the development of genetically modified mouse models.
Preece, Christopher, Davies, Benjamin, Hernandez-Pliego, Polinka, Bouchareb, Mohamed-Amine, Hinch, Robert, Alghadban, Samy, Biggs, Daniel   +6 more
core   +2 more sources

Diverse Systems for Efficient Sequence Insertion and Replacement in Precise Plant Genome Editing

BioDesign Research, 2020
CRISPR-mediated genome editing has been widely applied in plants to make uncomplicated genomic modifications including gene knockout and base changes.
Yingxiao Zhang, Yiping Qi
doaj   +1 more source

Genetically Engineered Organization: Protein Template, Biological Recognition Sites, and Nanoparticles

Advanced Materials Interfaces, 2016
Nanoparticles and their assemblies exhibit properties that can be used for a wide range of applications. However, creating multifunctional assemblies has remained challenging. Inspired by magnetotactic bacteria, genetically engineered single building blocks from magnetosome chains are used and complemented by additional components to form fluorescent ...
Jehle, F., Valverde-Tercedor, C., Reichel, V., Carillo, M., Bennet, M., Günther, E., Wirth, R., Mickoleit, F., Zarivach, R., Schüler, D., Blank, K., Faivre, D.   +11 more
openaire   +4 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source