Results 181 to 190 of about 13,537 (210)

MAb Targeting a Link Between ExoN and MTase of TGEV NSP14

Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2023
Porcine transmissible gastroenteritis virus (TGEV) infection results in severe gastrointestinal disease manifesting vomiting, diarrhea in neonatal porcine, with extremely high mortality. Monoclonal antibody (MAb) specific to TGEV nonstructural protein (NSP)14 that contains two functional domains, exonuclease (ExoN) and methyltransferase (MTase) domains,
Lilan, Xie, Fang, Lin, Peiling, Dong, Yaoming, Li   +3 more
openaire   +2 more sources

p53- And ROS-Mediated AIF Pathway Involved in TGEV-Induced Apoptosis

Journal of Veterinary Medical Science, 2018
We previously demonstrated that transmissible gastroenteritis virus (TGEV) could induce apoptosis through caspase signaling. However, apoptosis was not completely prevented by caspases inhibitors, suggesting that there may be a caspase-independent pathway involved in TGEV-induced cell apoptosis.
Meiling Hong, Li Ding, Weihao Li
exaly   +4 more sources

An Overview of Successful TGEV Vaccination Strategies and Discussion on the Interrelationship between TGEV and PRCV

1994
Porcine respiratory coronavirus (PRCV) is a new variant of TGE with an altered pathogenesis. PRCV multiplies mainly in tonsilar tissues and the respiratory tract. There are no enteric symptoms and in experimentally infected pigs, even the respiratory tract infection is usually asymptomatic.
M W, Welter, M P, Horstman, C J, Welter, L M, Welter   +3 more
openaire   +2 more sources

Control of TGEV mRNA Transcription

1994
Coronavirus proteins are translated from a nested set of subgenomic mRNAs which have common 3' termini with unique 5' extensions. Evidence suggests that coronavirus mRNAs are generated by a mechanism of leader primed transcription. Leader RNA binds to consensus sequences upstream of each gene on full length negative strand viral RNA and transcription ...
J, Hiscox, D H, Pocock, P, Britton
openaire   +2 more sources

Immune response of sows vaccinated with attenuated transmissible gastroenteritis virus (TGEV) and recombinant TGEV spike protein vaccines and protection of their suckling pigs against virulent TGEV challenge exposure

American Journal of Veterinary Research, 1998
Abstract Objective To compare recombinant transmissible gastroenteritis virus (TGEV) spike protein, (SP) R2-2, with attenuated live virus (ALV) vaccine in sows during late pregnancy. Animals 13 TGEV-seronegative sows and their pigs. Procedure At prepartum weeks (PPW) 6 and 4, sows of groups 1 and 2 received ALV via the oral/intranasal (O/IN) route.
S, Park, K, Sestak, D C, Hodgins, D I, Shoup, L A, Ward, D J, Jackwood, L J, Saif   +6 more
openaire   +2 more sources

Characterization of the Transmissible Gastroenteritis Virus (TGEV) Transcription Initiation Sequence

1995
The ability of the TGEV transcription initiation sequence (TIS) to produce subgenomic RNAs was investigated by placing a reporter gene, chloramphenicol acetyltransferase (CAT) under the control of either the mRNA 6 or the mRNA 7 TISs. Both constructs only produced CAT in TGEV infected cells and the amount of CAT produced from the mRNA 7 TIS was less ...
J A, Hiscox, K L, Mawditt, D, Cavanagh, P, Britton   +3 more
openaire   +2 more sources

Inhibition of Porcine Transmissible Gastroenteritis Virus (TGEV) Replication in Mini-Pigs by shRNA

Virus Research, 2010
Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis (TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine industry. Previously, we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA ...
Daoliang Lan, Zhibiao Yang
exaly   +3 more sources

The Genome of Transmissible Gastroenteritis Virus (TGEV)

1984
Lysis of isolated TGE virions with sodium dodecyl sulphate (SDS) at room temperature followed by electrophoresis, yields predominantly a single band of RNA running at about molecular weight 6.8 × 106. Heating the SDS lysate at 80°C for 15 min prior to electrophoresis results in a cluster of bands of between 0.2–6.8 × 106.
Lynne Bountiff, David J. Garwes, Geoffrey C. Millson, G. David Baird   +3 more
openaire   +1 more source

Expression of TGEV Structural Genes in Virus Vectors

1990
TGEV is a Coronavirus that causes gastroenteritis in pigs, resulting in a high mortality of neonates. The TGEV virion contains three major structural polypeptides; a surface glycoprotein (spike or peplomer protein) with a monomeric Mr 200000, a glycosylated integral membrane protein observed as a series of polypeptides of Mr 28000–31000 and a basic ...
D J, Pulford, P, Britton, K W, Page, D J, Garwes   +3 more
openaire   +2 more sources

Antigenic variation among transmissible gastroenteritis virus (tgev) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the S protein of tgev

American Journal of Veterinary Research, 1992
Summary Five nonneutralizing monoclonal antibodies (MAb) generated to the virulent Miller strain of transmissible gastroenteritis virus (tgev) and specific for the S protein were characterized. Competition assays between purified and biotinylated MAb indicated that MAb 75B10 and 8G11 mapped near a new subsite, designated V and 2 MAb, 44C11 and 45A8 ...
R A, Simkins, P A, Weilnau, J, Bias, L J, Saif   +3 more
openaire   +2 more sources