Results 251 to 260 of about 1,963,376 (294)

Studies on the thermal denaturation of nucleohistones

Journal of Molecular Biology, 1973
Abstract The thermal denaturation profiles of nucleohistone from calf thymus, sea urchin sperm and sea cucumber male gonad, are studied and compared under a variety of conditions. These include melting in the presence of either one of the following agents: urea, methanol, divalent cations or excess histones.
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Thermal Denaturation Assays in Chemical Biology

ASSAY and Drug Development Technologies, 2012
Thermal denaturation-based methods are becoming increasingly used to characterize protein stability and interactions. Recent technical advances have made these methods more suitable for high throughput screening. Reasonable throughput and the ability to perform these screens using commonly used instruments, such as RT-PCR machines or simple plate ...
Guillermo, Senisterra, Irene, Chau, Masoud, Vedadi   +2 more
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Stochastic dynamics and denaturation of thermalized DNA

Physical Review E, 2008
In the first part of the paper, the stochastic dynamics of the Peyrard-Bishop-Dauxois (PBD) DNA model is studied. A one-dimensional averaged Itô stochastic differential equation governing the total energy of the system and the associated Fokker-Planck equation governing the transition probability density function of the total energy are derived from ...
Mao Lin, Deng, Wei Qiu, Zhu
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Reversible thermal denaturation of Aplysia myoglobin

Journal of Molecular Biology, 1968
Abstract Ferric Aplysia myoglobin undergoes a rapidly reversible thermal denaturation in the range of 55 to 70 °C. In salt-free solution the high-temperature form precipitates, while in the presence of salts (ionic strength approx. 0.1 or higher) it remains soluble. The thermal transition, as studied in salt solution, is associated with large changes
M, Brunori, E, Antonini, P, Fasella, J, Wyman, A R, Fanelli   +4 more
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CALORIMETRIC INVESTIGATION OF RIBONUCLEASE THERMAL DENATURATION

International Journal of Peptide and Protein Research, 1973
Thermal denaturation of ribonuclease has been investigated by scanning microcalorimetric, spectrophotometric and polarimetric techniques. It is shown that the temperature changes of ribonuclease are of a complicated character and may be subdivided by the temperature region into two qualitatively different stages: (a) the predenaturational stage, at ...
P L, Privalov, E I, Tiktopulo, N N, Khechinashvili   +2 more
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The effect of thermal denaturation on the antigenicity of glycinin

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971
Abstract The effect of temperature and time of heating on the antigenicity of glycinin was investigated by the methods of radial immunodiffusion, complement fixation, and disc immunoelectrophoresis. The protein retains its immunological activity by heating for 30 min even up to 65°. However, a rapid loss in antigenicity is observed in the temperature
N, Catsimpoolas, J, Kenney, E W, Meyer
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Antigenicity of thermal denatured yoshida glycoprotein

Experientia, 1976
The correlation between antigenic functionality and conformation of Yoshida glycoprotein upon heating has been investigated. Heating modifies the conformation of the antigen, but does not abolish the reaction with its specific antibody.
A, Floridi, A, Caputo
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Effect of diethylsulfoxide on the thermal denaturation of DNA

Biopolymers, 2006
AbstractDNA thermal denaturation has been investigated in aqueous solutions of diethylsulfoxide (DESO) by means of UV‐vis and densimetry methods. It is suggested that, on the one hand, the structural change of entire solutions and, on the other hand, a direct interaction of DESO with DNA are responsible for the observed peculiar behavior.
Shiraz A, Markarian, Arpine M, Asatryan, Karine R, Grigoryan, Hasmik R, Sargsyan   +3 more
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The thermal denaturation of chromatin core particles

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
The thermal denaturation of chicken erythrocyte core particles has been followed using changes in absorption and circular dichroism. The absorption-denaturation curve is biphasic, with transitions centred at 59 degrees C and 74 degrees C. The first of these transitions is reversible, whereas heating into the second transition produces irreversible ...
I O, Walker, A P, Wolffe
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The thermal denaturation of nucleoprotein in boar sperm

Journal of Molecular Biology, 1965
The paper describes a study of the thermal denaturation of DNA in intact boar sperm. A recently designed microspectrophotometer was used to measure the integrated absorption at 260 m μ of sperm after they had been heated in several media. Denaturation was detected with heating media containing 70% glycerol and 30% of a sodium chloride-sodium citrate ...
P J, CHAMBERLAIN, P M, WALKER
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