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Antigenicity of thermal denatured yoshida glycoprotein
Experientia, 1976The correlation between antigenic functionality and conformation of Yoshida glycoprotein upon heating has been investigated. Heating modifies the conformation of the antigen, but does not abolish the reaction with its specific antibody.
A, Floridi, A, Caputo
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High-resolution analyzer of thermal denaturation
Analytical Biochemistry, 1974Abstract Details are given for the construction of a high-resolution denaturation analyzer for nucleic acid-containing macromolecules. The system contains the following new components: Peltier elements, guided by a linear resistance thermometer for temperature control, electronic microstirrer for quick thermal equilibration within the sample cell ...
P, Näslund +2 more
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Thermal Denaturation Assays in Chemical Biology
ASSAY and Drug Development Technologies, 2012Thermal denaturation-based methods are becoming increasingly used to characterize protein stability and interactions. Recent technical advances have made these methods more suitable for high throughput screening. Reasonable throughput and the ability to perform these screens using commonly used instruments, such as RT-PCR machines or simple plate ...
Guillermo, Senisterra +2 more
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Thermal denaturation of lobster hemocyanin
Biochimica et Biophysica Acta, 1957Abstract 1. 1. A procedure for the determination of heat denaturation of hemocyanin by means of changes in specific refractive increment has been presented. The method possesses the advantage of requiring a small volume (0.1 ml) of solution for analysis. 2. 2.
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Thermal Fluctuations in Histone During Denaturation
Journal of Nanoscience and Nanotechnology, 2007In this paper, we address the issue of thermal fluctuations during the thermal denaturation of linker histone H1 which is the basic ingredient of chromatin assembly. We measure the thermal fluctuations using a sensitive nanocalorimeter based thermal fluctuation measurement set up which can measure fluctuations of the order of 1 part per billion.
Nagapriya, KS +2 more
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Ligand-induced biphasic thermal denaturation of RNAase A
Journal of Thermal Analysis, 1994DSC measurements have been accomplished in aqueous solutions of bovine pancreatic ribonuclease A (RNAase A) in the presence of subsaturating amounts of 3′ cytidine monophosphate (3′ CMP) and 2′ cytidine monophosphate (2′ CMP) atpH 5.0 and 5.5. In these conditions the experimental profiles do not conform to a one-step unfolding process.
G. BARONE +5 more
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Thermal denaturation of collagen revisited
Proceedings / Indian Academy of Sciences, 1999We have recently re-examined the characteristic sharp denaturation temperature of the collagen molecule and fibre. It has been generally accepted for many years that denaturation is an equilibrium process involving the rupture of hydrogen bonds. We have now proposed that the process is an irreversible rate process, in which uncoupling of thea-chains ...
Christopher A Miles, Allen J Bailey
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Visualization of thermally denatured nucleosomes
Proceedings, annual meeting, Electron Microscopy Society of America, 1978Electron images of biological specimens composed of periodic structures are generally spurious. The reason is that unit structures may superimpose along the direction of view, and a “see through” image is formed especially from negatively stained and unstained objects.
N. H. Poon, V. L. Seligy
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Stochastic dynamics and denaturation of thermalized DNA
Physical Review E, 2008In the first part of the paper, the stochastic dynamics of the Peyrard-Bishop-Dauxois (PBD) DNA model is studied. A one-dimensional averaged Itô stochastic differential equation governing the total energy of the system and the associated Fokker-Planck equation governing the transition probability density function of the total energy are derived from ...
Mao Lin, Deng, Wei Qiu, Zhu
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Monitoring protein denaturation using thermal conductivity probe
International Journal of Biological Macromolecules, 2013We propose a method for probing denaturation of proteins by measuring the thermal conductivity of the solution. We use the three-omega method with a microfabricated ac thermal sensor to measure the thermal conductivity of lysozyme, β-lactoglobulin, and bovine serum albumin protein solutions over a range of temperature and pH.
Byoung kyoo Park +3 more
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