Results 251 to 260 of about 116,561 (299)

Thymus deoxyribonucleoproteinIV. Thermal denaturation

Biochimica et Biophysica Acta, 1963
Abstract The effects of thermal denaturation on the ultraviolet absorption and viscosity of calf-thymus deoxyribonucleohistone and deoxyribonucleic acid have been compared. The denaturation temperature of nucleohistone was higher than that of nucleic acid.
M F, LEE, I O, WALKER, A R, PEACOCKE
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Reversible thermal denaturation of Aplysia myoglobin

Journal of Molecular Biology, 1968
Abstract Ferric Aplysia myoglobin undergoes a rapidly reversible thermal denaturation in the range of 55 to 70 °C. In salt-free solution the high-temperature form precipitates, while in the presence of salts (ionic strength approx. 0.1 or higher) it remains soluble. The thermal transition, as studied in salt solution, is associated with large changes
M, Brunori, E, Antonini, P, Fasella, J, Wyman, A R, Fanelli   +4 more
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Thermal denaturation of lobster hemocyanin

Biochimica et Biophysica Acta, 1957
Abstract 1. 1. A procedure for the determination of heat denaturation of hemocyanin by means of changes in specific refractive increment has been presented. The method possesses the advantage of requiring a small volume (0.1 ml) of solution for analysis. 2. 2.
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The effect of thermal denaturation on the antigenicity of glycinin

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1971
Abstract The effect of temperature and time of heating on the antigenicity of glycinin was investigated by the methods of radial immunodiffusion, complement fixation, and disc immunoelectrophoresis. The protein retains its immunological activity by heating for 30 min even up to 65°. However, a rapid loss in antigenicity is observed in the temperature
N, Catsimpoolas, J, Kenney, E W, Meyer
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Studies on the thermal denaturation of nucleohistones

Journal of Molecular Biology, 1973
Abstract The thermal denaturation profiles of nucleohistone from calf thymus, sea urchin sperm and sea cucumber male gonad, are studied and compared under a variety of conditions. These include melting in the presence of either one of the following agents: urea, methanol, divalent cations or excess histones.
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Stochastic dynamics and denaturation of thermalized DNA

Physical Review E, 2008
In the first part of the paper, the stochastic dynamics of the Peyrard-Bishop-Dauxois (PBD) DNA model is studied. A one-dimensional averaged Itô stochastic differential equation governing the total energy of the system and the associated Fokker-Planck equation governing the transition probability density function of the total energy are derived from ...
Mao Lin, Deng, Wei Qiu, Zhu
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Kinetics of thermal denaturation of antithrombin III

Biotechnology and Bioengineering, 1982
AbstractPurified antithrombin III (AT III), a single‐chain human plasma glycoprotein, molecular weight 58,000 daltons, and one of the major serine protease inhibitors, was heated in the 60–70°C range for inactivating possible contaminations by hepatitis B virus (HBV).
G, Mitra, P M, Schneider, J L, Lundblad
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Microscopic description of DNA thermal denaturation

Applied Mathematics and Computation, 2019
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Mateusz Debowski, Miroslaw Lachowicz, Zuzanna Szymanska   +2 more
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Thermal Denaturation Assays in Chemical Biology

ASSAY and Drug Development Technologies, 2012
Thermal denaturation-based methods are becoming increasingly used to characterize protein stability and interactions. Recent technical advances have made these methods more suitable for high throughput screening. Reasonable throughput and the ability to perform these screens using commonly used instruments, such as RT-PCR machines or simple plate ...
Guillermo, Senisterra, Irene, Chau, Masoud, Vedadi   +2 more
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High-resolution analyzer of thermal denaturation

Analytical Biochemistry, 1974
Abstract Details are given for the construction of a high-resolution denaturation analyzer for nucleic acid-containing macromolecules. The system contains the following new components: Peltier elements, guided by a linear resistance thermometer for temperature control, electronic microstirrer for quick thermal equilibration within the sample cell ...
P, Näslund, B, Liljesvan, L, Abrahamsson   +2 more
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