Results 251 to 260 of about 116,006 (295)

Microscopic description of DNA thermal denaturation

Applied Mathematics and Computation, 2019
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Mateusz Debowski, Miroslaw Lachowicz, Zuzanna Szymanska   +2 more
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Thermal Denaturation Assays in Chemical Biology

ASSAY and Drug Development Technologies, 2012
Thermal denaturation-based methods are becoming increasingly used to characterize protein stability and interactions. Recent technical advances have made these methods more suitable for high throughput screening. Reasonable throughput and the ability to perform these screens using commonly used instruments, such as RT-PCR machines or simple plate ...
Guillermo, Senisterra, Irene, Chau, Masoud, Vedadi   +2 more
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Studies on the thermal denaturation of nucleohistones

Journal of Molecular Biology, 1973
Abstract The thermal denaturation profiles of nucleohistone from calf thymus, sea urchin sperm and sea cucumber male gonad, are studied and compared under a variety of conditions. These include melting in the presence of either one of the following agents: urea, methanol, divalent cations or excess histones.
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Stochastic dynamics and denaturation of thermalized DNA

Physical Review E, 2008
In the first part of the paper, the stochastic dynamics of the Peyrard-Bishop-Dauxois (PBD) DNA model is studied. A one-dimensional averaged Itô stochastic differential equation governing the total energy of the system and the associated Fokker-Planck equation governing the transition probability density function of the total energy are derived from ...
Mao Lin, Deng, Wei Qiu, Zhu
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Kinetics of thermal denaturation of antithrombin III

Biotechnology and Bioengineering, 1982
AbstractPurified antithrombin III (AT III), a single‐chain human plasma glycoprotein, molecular weight 58,000 daltons, and one of the major serine protease inhibitors, was heated in the 60–70°C range for inactivating possible contaminations by hepatitis B virus (HBV).
G, Mitra, P M, Schneider, J L, Lundblad
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High-resolution analyzer of thermal denaturation

Analytical Biochemistry, 1974
Abstract Details are given for the construction of a high-resolution denaturation analyzer for nucleic acid-containing macromolecules. The system contains the following new components: Peltier elements, guided by a linear resistance thermometer for temperature control, electronic microstirrer for quick thermal equilibration within the sample cell ...
P, Näslund, B, Liljesvan, L, Abrahamsson   +2 more
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Antigenicity of thermal denatured yoshida glycoprotein

Experientia, 1976
The correlation between antigenic functionality and conformation of Yoshida glycoprotein upon heating has been investigated. Heating modifies the conformation of the antigen, but does not abolish the reaction with its specific antibody.
A, Floridi, A, Caputo
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Effect of diethylsulfoxide on the thermal denaturation of DNA

Biopolymers, 2006
AbstractDNA thermal denaturation has been investigated in aqueous solutions of diethylsulfoxide (DESO) by means of UV‐vis and densimetry methods. It is suggested that, on the one hand, the structural change of entire solutions and, on the other hand, a direct interaction of DESO with DNA are responsible for the observed peculiar behavior.
Shiraz A, Markarian, Arpine M, Asatryan, Karine R, Grigoryan, Hasmik R, Sargsyan   +3 more
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Immunochemical studies on thermal denaturation of ovomucoid

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
The thermal denaturation of ovomucoid was investigated by immunochemical methods, namely immunoprecipitation analyses and antibody-Sepharose 4B column chromatography. In the immunoprecipitation analyses, heated ovomucoid (90 degrees C, 90 min, pH 7.2) required about twice the antigen addition of the native protein to approach maximal precipitation with
T, Matsuda, K, Watanabe, R, Nakamura
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CALORIMETRIC INVESTIGATION OF RIBONUCLEASE THERMAL DENATURATION

International Journal of Peptide and Protein Research, 1973
Thermal denaturation of ribonuclease has been investigated by scanning microcalorimetric, spectrophotometric and polarimetric techniques. It is shown that the temperature changes of ribonuclease are of a complicated character and may be subdivided by the temperature region into two qualitatively different stages: (a) the predenaturational stage, at ...
P L, Privalov, E I, Tiktopulo, N N, Khechinashvili   +2 more
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