Results 261 to 270 of about 116,561 (299)
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Antigenicity of thermal denatured yoshida glycoprotein
Experientia, 1976The correlation between antigenic functionality and conformation of Yoshida glycoprotein upon heating has been investigated. Heating modifies the conformation of the antigen, but does not abolish the reaction with its specific antibody.
A, Floridi, A, Caputo
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Effect of diethylsulfoxide on the thermal denaturation of DNA
Biopolymers, 2006AbstractDNA thermal denaturation has been investigated in aqueous solutions of diethylsulfoxide (DESO) by means of UV‐vis and densimetry methods. It is suggested that, on the one hand, the structural change of entire solutions and, on the other hand, a direct interaction of DESO with DNA are responsible for the observed peculiar behavior.
Shiraz A, Markarian +3 more
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CALORIMETRIC INVESTIGATION OF RIBONUCLEASE THERMAL DENATURATION
International Journal of Peptide and Protein Research, 1973Thermal denaturation of ribonuclease has been investigated by scanning microcalorimetric, spectrophotometric and polarimetric techniques. It is shown that the temperature changes of ribonuclease are of a complicated character and may be subdivided by the temperature region into two qualitatively different stages: (a) the predenaturational stage, at ...
P L, Privalov +2 more
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Immunochemical studies on thermal denaturation of ovomucoid
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982The thermal denaturation of ovomucoid was investigated by immunochemical methods, namely immunoprecipitation analyses and antibody-Sepharose 4B column chromatography. In the immunoprecipitation analyses, heated ovomucoid (90 degrees C, 90 min, pH 7.2) required about twice the antigen addition of the native protein to approach maximal precipitation with
T, Matsuda, K, Watanabe, R, Nakamura
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Effects of thermal denaturation on protein glycation
Life Sciences, 2002Protein denaturation occurs at sites of inflammation. We hypothesized that denatured protein may provide a more susceptible target for glycation, which is a known mediator of inflammation. We examined the effects of thermal denaturation on the susceptibility of protein glycation using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and aspartate ...
Norbert W, Seidler, George S, Yeargans
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The thermal denaturation of nucleoprotein in boar sperm
Journal of Molecular Biology, 1965The paper describes a study of the thermal denaturation of DNA in intact boar sperm. A recently designed microspectrophotometer was used to measure the integrated absorption at 260 m μ of sperm after they had been heated in several media. Denaturation was detected with heating media containing 70% glycerol and 30% of a sodium chloride-sodium citrate ...
P J, CHAMBERLAIN, P M, WALKER
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The thermal denaturation of chromatin core particles
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984The thermal denaturation of chicken erythrocyte core particles has been followed using changes in absorption and circular dichroism. The absorption-denaturation curve is biphasic, with transitions centred at 59 degrees C and 74 degrees C. The first of these transitions is reversible, whereas heating into the second transition produces irreversible ...
I O, Walker, A P, Wolffe
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Ligand-induced biphasic thermal denaturation of RNAase A
Journal of Thermal Analysis, 1994DSC measurements have been accomplished in aqueous solutions of bovine pancreatic ribonuclease A (RNAase A) in the presence of subsaturating amounts of 3′ cytidine monophosphate (3′ CMP) and 2′ cytidine monophosphate (2′ CMP) atpH 5.0 and 5.5. In these conditions the experimental profiles do not conform to a one-step unfolding process.
G. BARONE +5 more
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Thermal denaturation profiles and the structure of chromatin
Nature, 1976Analysis of the melting profile of DNA stripped of non-histone proteins and histone H1 allows definite structural conclusions to be drawn; conversely, it is possible to calculate what a particular chromatin model's melting curve will look like.
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A SIMPLE MODEL FOR PROTEIN THERMAL DENATURATION*
International Journal of Peptide and Protein Research, 1978Whether proteins denature in an all‐or‐none fashion or in a continuous fashion is as yet an unresolved problem. The all‐or‐none process implies that while the process of denaturation is going on, only two kinds of protein molecules can exist. One is completely unchanged and the other is altered.
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