Results 201 to 210 of about 6,447 (219)
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Journal of Proteomics, 2021
Trypanosoma cruzi is a flagellate protozoa being the etiological agent of Chagas disease, a neglected tropical disease, which still poses a public health problem worldwide. The intricate molecular changes during T. cruzi-host interaction have been explored using different largescale omics techniques.
Joao V P, Coutinho +9 more
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Trypanosoma cruzi is a flagellate protozoa being the etiological agent of Chagas disease, a neglected tropical disease, which still poses a public health problem worldwide. The intricate molecular changes during T. cruzi-host interaction have been explored using different largescale omics techniques.
Joao V P, Coutinho +9 more
openaire +2 more sources
Discovery of metal-binding proteins by thermal proteome profiling
Nature Chemical BiologyMetal-binding proteins (MBPs) have various and important biological roles in all living species and many human diseases are intricately linked to dysfunctional MBPs. Here, we report a chemoproteomic method named 'metal extraction-triggered agitation logged by thermal proteome profiling' (METAL-TPP) to globally profile MBPs in proteomes.
Xin Zeng +13 more
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PACTS-Assisted Thermal Proteome Profiling for Use in Identifying Peptide-Interacting Proteins
Analytical Chemistry, 2022Bioactive peptides play important roles in various biological processes. However, the traditional methods for profiling the peptide-interacting proteins require modifications to the peptide molecules, often leading to false identifications. We found that the interaction between peptide ligands and protein receptors induced significant changes in the ...
Ting Zhao +8 more
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Thermal proteome profiling monitors ligand interactions with cellular membrane proteins
Nature Methods, 2015We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins.
Friedrich B M Reinhard +10 more
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Effect of Drug-to-Protein Reaction Kinetics on the Results of Thermal Proteome Profiling
Analytical ChemistryIn this Letter, a two-term formalism for constructing protein solubility curves in thermal proteome profiling (TPP) is considered, which takes into account the efficiency of the drug-protein binding reaction. When the reaction is incomplete, this results in distortion of the otherwise sigmoidal shape of the curve after drug treatment, which is often ...
Ivan I. Fedorov +2 more
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Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkers
2022Abstract The complexity of the functional proteome extends significantly beyond the protein coding genome resulting in millions of proteoforms. Investigation of proteoforms and their functional roles is important to understand cellular physiology and its deregulation in diseases, but challenging to perform systematically. Here, we apply
Nils Kurzawa +10 more
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[Thermal proteome profiling: a technique for a comprehensive assessment of protein status].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2022Thermal proteome profiling (TPP) is a combination of cellular thermal shift assay (CETSA) and quantitative mass spectrometry (MS), also termed as MS-CETSA. TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures. Proteins can change their thermostability
Yanhua, Qiu +4 more
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Analytical and Bioanalytical Chemistry
Advances in high-throughput high-resolution mass spectrometry and the development of thermal proteome profiling approach (TPP) have made it possible to accelerate a drug target search. Since its introduction in 2014, TPP quickly became a method of choice in chemical proteomics for identifying drug-to-protein interactions on a proteome-wide scale and ...
Ivan I. Fedorov +8 more
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Advances in high-throughput high-resolution mass spectrometry and the development of thermal proteome profiling approach (TPP) have made it possible to accelerate a drug target search. Since its introduction in 2014, TPP quickly became a method of choice in chemical proteomics for identifying drug-to-protein interactions on a proteome-wide scale and ...
Ivan I. Fedorov +8 more
openaire +2 more sources
Thermal Proteome Profiling Identifies the Target of Indophagolin
Synfacts, 2021openaire +1 more source