Thermal proteome profiling: Insights into protein modifications, associations, and functions
Current Opinion in Chemical Biology, 2022Tracking proteins' biophysical characteristics on a proteome-wide scale can provide valuable information on their functions and interactions. Thermal proteome profiling (TPP) is a multiplexed quantitative proteomics approach that measures changes in protein thermal stability-a key biophysical property-across different cellular states. Developed in 2014,
Henrik Hammaren +2 more
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A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand
Proteomics, 2020AbstractThermal proteome profiling is a powerful energetic‐based chemical proteomics method to reveal the ligand‐protein interaction. However, the costly multiplexed isotopic labeling reagent, mainly Multiplexed isobaric tandem mass tag (TMT), and the long mass spectrometric time limits the wide application of this method.
Yanan Li, Lianghai Hu, Xiaolei Zhang
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Tracking cancer drugs in living cells by thermal profiling of the proteome
Science, 2014Mapping human drug targets in the cell To understand both the beneficial and the side effects of a drug, one would need to know its full binding profile to all cellular proteins. Savitski et al. take significant steps toward meeting this daunting challenge.
Dirk Eberhard +2 more
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Thermal proteome profiling identifies the membrane-bound purinergic receptor P2X4 as a target of the autophagy inhibitor indophagolin [PDF]
Cell Chemical Biology, 2021 Signaling pathways are frequently activated through signal-receiving membrane proteins, and the discovery ofsmall molecules targeting these receptors may yield insights into their biology.
Luca Laraia +2 more
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Drug Target Identification in Tissues by Thermal Proteome Profiling
Annual Review of Pharmacology and Toxicology, 2022Drug target deconvolution can accelerate the drug discovery process by identifying a drug's targets (facilitating medicinal chemistry efforts) and off-targets (anticipating toxicity effects or adverse drug reactions). Multiple mass spectrometry–based approaches have been developed for this purpose, but thermal proteome profiling (TPP) remains to date ...
André, Mateus +4 more
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Proteomic profiling of thermal acclimation in Drosophila melanogaster
Insect Biochemistry and Molecular Biology, 2013Thermal acclimation drastically alters thermotolerance of ectotherms, but the mechanisms determining this plastic response are not fully understood. The present study investigates the proteomic response (2D-DIGE) of adult Drosophila melanogaster acclimated at 11, 25 or 31 °C.
Colinet, Hervé +3 more
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Thermal proteome profiling reveals the glial toxicity of dencichine via inhibiting proteasome
Food and Chemical Toxicology, 2023Dezhi Kong
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Identifying drug targets with thermal proteome profiling using IBT‐16plex
Rapid Communications in Mass Spectrometry, 2023Rationale Thermal proteome profiling (TPP) has been widely used for the identification of drug targets for several years, and TMTpro‐16plex has recently been evaluated for TPP of vehicle‐ and drug‐treated samples in a single labeling process to reduce missing values and save instrument time.
Zhaomei Shi +3 more
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Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States
2023Proteins are central drivers of physiological and pathological processes in the cell. Methods evaluating protein functional states are therefore vital to fundamental research as well as drug discovery. Thermal proteome profiling (TPP) to this date constitutes the only approach that permits examining protein states in live cells, under native conditions
Patricia Sauer, Marcus Bantscheff
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Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling
Analytical ChemistryTarget deconvolution is essential for elucidating the molecular mechanisms, therapeutic efficacy, and off-target toxicity of small-molecule drugs. Thermal proteome profiling (TPP) is a robust and popular method for identifying drug-protein interactions.
Qiqi Wang +5 more
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