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Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity

Biotechnology Letters, 2019
The biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCITase) expressed in Escherichia coli and purified to a single protein.The 171-kDa protein displayed maximum activity at pH 6.0, and enzyme activity was ...
So-Jin, Yang   +3 more
openaire   +2 more sources

Establishment of a Genetic Transformation System and Its Application in Thermoanaerobacter tengcongensis

Journal of Genetics and Genomics, 2012
The whole-genome sequence of Thermoanaerobacter tengcongensis, an anaerobic thermophilic bacterium isolated from the Tengchong hot spring in China, was completed in 2002. However, in vivo studies on the genes of this strain have been hindered in the absence of genetic manipulation system.
Bo, Liu   +3 more
openaire   +2 more sources

Proteomic analysis on the temperature‐dependent complexes in Thermoanaerobacter tengcongensis

PROTEOMICS, 2009
AbstractIt is generally accepted that protein complexes play an active role in avoiding the protein degradation of the thermophiles. Thermoanaerobacter tengcongensis was cultured at three different temperatures (55, 75 and 80°C) and the extracts of protein complexes were prepared.
Bo, Meng   +11 more
openaire   +2 more sources

Characterization of the impact of acetate and lactate on ethanolic fermentation by Thermoanaerobacter ethanolicus

Bioresource Technology, 2009
Ethanolic fermentation of simple sugars is an important step in the production of bioethanol as a renewable fuel. Significant levels of organic acids, which are generally considered inhibitory to microbial metabolism, could be accumulated during ethanolic fermentation, either as a fermentation product or as a by-product generated from pre-treatment ...
Qiang, He   +3 more
openaire   +2 more sources

Crystal structure of thermophilic dextranase fromThermoanaerobacter pseudethanolicus

Journal of Biochemistry, 2015
The crystal structures of the wild type and catalytic mutant Asp-312→Gly in complex with isomaltohexaose of endo-1,6-dextranase from the thermophilic bacterium Thermoanaerobacter pseudethanolicus (TpDex), belonging to the glycoside hydrolase family 66, were determined.
Nobuhiro, Suzuki   +8 more
openaire   +2 more sources

Crystal structure of scaffolding protein CheW from thermoanaerobacter tengcongensis

Biochemical and Biophysical Research Communications, 2007
The crystal structure of the scaffolding protein CheW from Thermoanaerobacter tengcongensis (TtCheW) is reported with a resolution at 2.2A using molecular replacement. Based on the crystal structure TmCheA P4-P5-TmCheW from Thermotoga maritime reported by others, we modeled the TmCheA P4-P5-TtCheW complex and predicted that TtCheW is involved in a ...
Wang, Yao, Liang, Shi, Dong-Cai, Liang
openaire   +2 more sources

Methods and Supports for Immobilization and Stabilization of Cyclomaltodextrin Glucanotransferase from Thermoanaerobacter

Applied Biochemistry and Biotechnology, 2007
Thermoanaerobacter cyclomaltodextrin glucanotransferase (CGTase) was immobilized using different supports and immobilization methods to study the effect on activity recovery. The enzyme covalently attached into glyoxyl-silica showed low activity recovery of 1.5%.
Ana Elisa, Amud   +5 more
openaire   +2 more sources

The mechanism for regulating ethanol fermentation by redox levels in Thermoanaerobacter ethanolicus

Metabolic Engineering, 2011
Anaerobes can obtain the entire cell's ATP by glycolysis and remove resulting reducing power by fermentation. There is a delicate balance in redox status to obtain a maximal growth of these cells, and the conditions to change redox fluxes can induce kinds of changes in metabolism.
Jianjun, Pei   +7 more
openaire   +2 more sources

[14] Chaperonins from Thermoanaerobacter species

1998
Robert K. Scopes, Kaye Truscott
openaire   +1 more source

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