Results 151 to 160 of about 38,411 (253)

Engineering of a Carbonic Anhydrase from <i>Hydrogenimonas thermophila</i> Through Fusion Tags and Surface Mutagenesis Enhances Solubility While Revealing Stability-Function Relationships. [PDF]

Int J Mol Sci
Manyumwa CV, Jers C, Mijakovic I.
europepmc   +1 more source

ESMStabP: A Regression Model for Protein Thermostability Prediction [PDF]

Accurately predicting protein thermostability is crucial for numerous applications in biotechnology, pharmaceuticals, and food science. Experimental methods for determining protein melting temperatures are often time-consuming and costly, driving the ...
Jernigan, Robert, Ramos, Marcus, Kilinc, Mesih   +2 more
core  

Improving ARTs: Microfluidics as a Tool for Sperm Selection

Andrology, EarlyView.
ABSTRACT Sperm selection is a critical step in assisted reproductive technology (ART), as sperm quality directly influences embryo quality. In vivo, the female reproductive tract imposes several physical and physiological barriers that favors only the most optimal spermatozoa reaching the fertilization site.
A. Pérez‐Gómez, A. Urriés, J. L. de Pablo, L. Serrano‐Ramón, S. Sanchez, R. Herrer, J. M. Morrell, C. Malo   +7 more
wiley   +1 more source

Growth factor thermostability dataset derived from experimental and computational approaches to accelerate cultured meat development. [PDF]

Data Brief
Ng W, Aghakasiri K, Pierce R, Marschallinger J, Ong KJ, Rees SD, Staszak D, Duffy B.   +7 more
europepmc   +1 more source

Thermodynamic characterisation of covalent ligand binding

British Journal of Pharmacology, EarlyView.
Background and Purpose Differential scanning fluorimetry (DSF) is a common and straightforward method to evaluate the thermal stability of proteins and has been heavily used for ligand binding characterisation as well as for screening. One class of compounds that is less typically evaluated by DSF are covalent binders.
Rebecca Hertzman, Maayan Lavie, Almog Nadir, Ronen Gabizon, Nir London   +4 more
wiley   +1 more source

Corrigendum: Directed Evolution of Pseudomonas fluorescens Lipase Variants With Improved Thermostability Using Error-Prone PCR

Frontiers in Bioengineering and Biotechnology, 2020
Lijun Guan, Yang Gao, Jialei Li, Kunlun Wang, Zhihong Zhang, Song Yan, Nina Ji, Ye Zhou, Shuwen Lu   +8 more
doaj   +1 more source

Thermostability Engineering in Therapeutic Antioxidant Enzymes: From Molecular Fundamentals to Oxidative Stress Applications. [PDF]

Int J Mol Sci
Tatarciuc D, Esanu IM, Foia I, Trandafirescu MF, Vasilcu TF, Ghica DC, Antohe ME, Armencia AO, Vasluianu RI.   +8 more
europepmc   +1 more source

Structural insights into xyloglucan recognition by an ABC transporter from a Gram‐positive, thermophilic bacterium

The FEBS Journal, EarlyView.
Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo, Virginia Jiang, Philip D. Jeffrey, Angela Zhu, A. James Link, Jerelle A. Joseph, Jonathan M. Conway   +6 more
wiley   +1 more source

HA thermostability mutations S84F, G167N, and D168N potentiate H9N2 virus transmission in a warming environment. [PDF]

Emerg Microbes Infect
Pei X, Chen W, Xiao H, Ren B, Guo C, Gao X, Peng Z, Zhang L, Sun Q, Hu Z, Zhou H, Ma S, Li X, Su W.   +13 more
europepmc   +1 more source

Insights into tandem dioxygenases—copper radical oxidases in fungal Phytopathogens of the Colletotrichum acutatum species complex

The FEBS Journal, EarlyView.
In Colletotrichum acutatum species complex, conserved ring‐cleavage dioxygenases located adjacent to AA5_2 enzymes were investigated as potential redox partners. Biochemical analyses identified the dioxygenases as intradiol‐cleaving enzymes preferentially oxidizing methyl‐substituted catechols, while AA5_2 enzymes displayed weak alcohol oxidase ...
Radka Končitíková, David Ribeaucourt, Mireille Haon, David Navarro, Elodie Drula, Bastien Bissaro, Jean‐Guy Berrin, Mickael Lafond   +7 more
wiley   +1 more source