Results 21 to 30 of about 68,203 (335)

Thermostability Improvement of L-Asparaginase from Acinetobacter soli via Consensus-Designed Cysteine Residue Substitution

Molecules, 2022
To extend the application range of L-asparaginase in food pre-processing, the thermostability improvement of the enzyme is essential. Herein, two non-conserved cysteine residues with easily oxidized free sulfhydryl groups, Cys8 and Cys283, of ...
Linshu Jiao, Huibing Chi, Bingjie Xia, Zhaoxin Lu, Xiaomei Bie, Haizhen Zhao, Fengxia Lu, Meirong Chen   +7 more
doaj   +1 more source

Generalized dynamical thermostating technique [PDF]

Physical Review E, 2003
We demonstrate that the Nosé method for constant-temperature molecular-dynamics simulation [Mol. Phys. 52, 255 (1984)] can be substantially generalized by the addition of auxiliary variables to encompass an infinite variety of Hamiltonian thermostats.
Laird, Brian Bostian, Leimkuhler, Benedict J.   +1 more
openaire   +3 more sources

A family of thermostable fungal cellulases created by structure-guided recombination [PDF]

, 2009
SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermostable CBH II chimeras.
A. Villalobos, C. D. Snow, C. Nguyen, F. H. Arnold, Go, Godbole, Hui, I. Wu, Innis, J. Minshull, Jeoh, Koivula, Li, Liu, Lussier, Nelson, Otey, P. Heinzelman, S. Govindarajan, Sch  lein   +19 more
core   +3 more sources

Factors enhancing protein thermostability [PDF]

Protein Engineering, Design and Selection, 2000
Several sequence and structural factors have been proposed to contribute toward greater stability of thermophilic proteins. Here we present a statistical examination of structural and sequence parameters in representatives of 18 non-redundant families of thermophilic and mesophilic proteins.
S, Kumar, C J, Tsai, R, Nussinov
openaire   +2 more sources

Functional stability of HIV-1 envelope trimer affects accessibility to broadly neutralizing antibodies at its apex [PDF]

, 2017
The trimeric envelope glycoprotein spike (Env) of HIV-1 is the target of vaccine development to elicit broadly neutralizing antibodies (bnAbs). Env trimer instability and heterogeneity in principle make subunit interfaces inconsistent targets for the ...
Gift, Syna Kuriakose, Kim, Arthur S, Leaman, Daniel P, Zhang, Lei, Zwick, Michael B   +4 more
core   +2 more sources

Enzyme thermostability and thermoactivity [PDF]

"Protein Engineering, Design and Selection", 1996
Proteins from hyperthermophilic micro-organisms (living at >85°C) are thermostable in that they are usually able to withstand temperatures that would rapidly denature most proteins from mesophiles (living at 20-40°C) such as Escherichia coli or humans. In most cases, the stability is intrinsic to the protein, although in some hyperthermophilic Archaea ...
M J, Danson, D W, Hough, R J, Russell, G L, Taylor, L, Pearl   +4 more
openaire   +2 more sources

Myeloid p38 activation maintains macrophage–liver crosstalk and BAT thermogenesis through IL‐12–FGF21 axis

Hepatology, EarlyView., 2022
Physiological activation of myeloid p38 controls macrophage IL‐12 production and crosstalk to the liver by modulating hepatic FGF21, and subsequently, brown adipose tissue thermogenesis during obesity Abstract Obesity features excessive fat accumulation in several body tissues and induces a state of chronic low‐grade inflammation that contributes to ...
María Crespo, Ivana Nikolic, Alfonso Mora, Elena Rodríguez, Luis Leiva‐Vega, Aránzazu Pintor‐Chocano, Daniel Horrillo, Lourdes Hernández‐Cosido, Jorge L. Torres, Eva Novoa, Rubén Nogueiras, Gema Medina‐Gómez, Miguel Marcos, Magdalena Leiva, Guadalupe Sabio   +14 more
wiley   +1 more source

Effects of mutations in the helix G region of horseradish peroxidase [PDF]

, 2008
Horseradish peroxidase (HRP) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors and biocatalysis.
Barry J. Ryan, Batra, Chakrabarti, Chang, Chattopadhyay, Ciarán Ó'Fágáin, Coates, Dai, Dunford, French, Gajhede, Garcia, Gonera, Gorman, Grigorenko, Guex, Hakamada, Haque, Hassani, Howes, Ignatenko, Jager, Kaposi, Khmelnitsky, Krowarsch, Kwon, Lei, Lin, Lipovsek, Liu, Liu, Machado, Miland, Miland, Mogharrab, Moosavi-Movahedi, Morawski, Nagano, Numata, O'Brien, O'Brien, Orlova, Ozaki, Pina, Priori, Ryan, Ryan, Ryan, Ryu, Sambrook, Santucci, Savenkova, Smeller, Smith, Tams, Tanaka, Tanaka, Ugarova, Urrutigoity, Wang   +59 more
core   +1 more source

Partially thermostated Kac model [PDF]

Journal of Mathematical Physics, 2015
We study a system of N particles interacting through the Kac collision, with m of them interacting, in addition, with a Maxwellian thermostat at temperature 1β. We use two indicators to understand the approach to the equilibrium Gaussian state. We prove that (i) the spectral gap of the evolution operator behaves as mN for large N and (ii) the relative ...
Tossounian, Hagop, Vaidyanathan, Ranjini
openaire   +3 more sources

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source