Results 31 to 40 of about 38,411 (253)
Cellulase protein engineering towards improved thermostability
, 2021 Lignocellulosic biomass is a promising, abundant, and inexpensive raw material, which can be utilized in several industries such as pulp and paper, textiles, food, feed, and biofuels.
Contreras Leiva, Francisca
core +1 more source
Nature Communications, 2020 Thermostability of the peptide-MHC interaction is important for immunogenicity. Here the authors present a mass spectrometry method to measure thermostability among thousands of peptide-MHC complexes in parallel and a trained artificial neural network to
Emma C. Jappe +9 more
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Frontiers in Bioengineering and Biotechnology, 2021 The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important.
Yuki Ohmuro-Matsuyama +6 more
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In Silico Approach to Elucidate Factors Associated with GH1 β-Glucosidase Thermostability
, 2019 b-Glucosidase is a class of hydrolytic enzymes that catalyzes the removal of the non-reducing b-D-glucosyl unit from various disaccharides and substituted b-D-glucosides.
Aasia Bibi +4 more
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BMC Biotechnology, 2018 Background Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to withstand transient high ...
Nanyu Han +7 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2022 In order to improve enzymatic properties of glucoamylases, six recombinant genes GA1–GA6 were created by domain shuffling of glucoamylase genes GAA1 from Aspergillus niger Ld418AI and GATE from Talaromyces emersonii Ld418 TE using overlap extension PCR ...
Zhongxiu Chen +15 more
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A Probabilistic Method to Correlate Ion-pairs to Protein Thermostability [PDF]
, 2010 [[abstract]]Recent developments in research on the stability of proteins show that ion-pairs potentially contribute to the thermostability of proteins, according to comparisons of ion-pairs between homologous structures.
S. L. Huang;L. C. Wu;Laing, K. T. Pan;M. T. Ko;J. T. Horng
core
ThermoBRET: A Ligand-Engagement Nanoscale Thermostability Assay Applied to GPCRs** [PDF]
Measurements of membrane protein thermostability reflect ligand binding. Current thermostability assays often require protein purification or rely on pre-existing radiolabelled or fluorescent ligands, limiting their application to established targets ...
Holliday, Nicholas D. +10 more
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Smaller is better: nanobodies meet NMR
FEBS Letters, EarlyView.Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley +1 more source
Engineering Enzymes for Enhanced Thermostability
, 1990 Chicken egg-white lysozyme (CEWL) is used as a model to attempt to engineer proteins for enhanced thermostability. Site-directed mutagenesis is employed for selective amino acid substitution to probe the contribution of an individual amino acid in a ...
Phoebe Shih +2 more
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