Results 51 to 60 of about 55,424 (301)
An Engineered Living Material With Pro‐Angiogenic Activity Inducible by Near‐Infrared Light
Advanced Functional Materials, EarlyView.NIR‐responsive engineered living materials (ELMs) for controlled angiogenesis: Near‐infrared (800 nm) light activates engineered probiotic bacteria within alginate‐based living materials to secrete a blood vessel‐regenerating protein. The released protein promotes pro‐angiogenic effects in endothelial networks and chick chorioallantoic membranes.
Anwesha Chatterjee +4 more
wiley +1 more source
Acrylamide Production Using Encapsulated Nitrile Hydratase from \u3cem\u3ePseudonocardia thermophila\u3c/em\u3e in a Sol–gel Matrix [PDF]
, 2014 The cobalt-type nitrile hydratase from Pseudonocardia thermophila JCM 3095 (PtNHase) was successfully encapsulated in tetramethyl orthosilicate sol–gel matrices to produce a PtNHase:sol–gel biomaterial.
Elgren, Timothy E. +4 more
core +1 more source
Advanced Functional Materials, EarlyView.The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett +19 more
wiley +1 more source
Overproduction of Tk-Subtilisin and Elucidation of Its Structural Thermostability Based on Molecular Dynamics Studies [PDF]
Brazilian Archives of Biology and TechnologyBackground: Subtilisin from Thermococcus kodakaraensis (Tk-subtilisin) is an ideal candidate for many industrial and research processes due to its unique features. Methods: In this study, the enzyme is overproduced in E.
Nouman Rasool, Naeem Rashid
doaj +1 more source
Microorganisms, 2021 This study analyzed the thermostability and effect of calcium ions on the enzymatic activity of α-amylase produced by Bacillus licheniformis strain LB04 isolated from Espinazo Hot springs in Nuevo Leon, Mexico. The enzyme was immobilized by entrapment on
Anaid Silva-Salinas +5 more
doaj +1 more source
Machine learning-guided directed evolution for protein engineering [PDF]
, 2019 Machine learning (ML)-guided directed evolution is a new paradigm for biological design that enables optimization of complex functions. ML methods use data to predict how sequence maps to function without requiring a detailed model of the underlying ...
Arnold, Frances H. +2 more
core +1 more source
Assaying activity and assessing thermostability of hyperthermophilic enzymes [PDF]
, 2001 There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature.
Akanuma +22 more
core +2 more sources
Advanced Materials, EarlyView.A cryoprotectant‐compatible nanoporous platform enables ambient‐stable and scalable delivery of gene editing therapeutics. By combining hierarchical pore architecture with optimized lyophilization chemistry, the system preserves Cas9‐RNP activity post‐freeze‐drying.
Sian Lee +8 more
wiley +1 more source
Thermostable and Long-Circulating Albumin-Conjugated Arthrobacter globiformis Urate Oxidase
Pharmaceutics, 2021 Urate oxidase derived from Aspergillus flavus has been investigated as a treatment for tumor lysis syndrome, hyperuricemia, and gout. However, its long-term use is limited owing to potential immunogenicity, low thermostability, and short circulation time
Byungseop Yang, Inchan Kwon
doaj +1 more source
Phase Engineering of Atomically Precise Nanoclusters (APNCs) of Gold and Beyond
Advanced Materials, EarlyView.Engineering the structural phase of materials is of paramount importance for both fundamental research and practical applications. In this Review, we summarize the recent progress in controlling the phases of atomically precise nanoclusters (APNCs) of gold, silver and copper, as well as bimetallic systems. The phase‐enabled material properties of APNCs
Yitong Wang +4 more
wiley +1 more source