Results 61 to 70 of about 55,424 (301)
Parallel Strategy Increases the Thermostability and Activity of Glutamate Decarboxylase
Molecules, 2020 Glutamate decarboxylase (GAD; EC 4.1.1.15) is a unique pyridoxal 5-phosphate (PLP)-dependent enzyme that specifically catalyzes the decarboxylation of L-glutamic acid to produce γ-aminobutyric acid (GABA), which exhibits several well-known ...
Qing-Fei Zhang +5 more
doaj +1 more source
Foods, 2023 Proteases have been widely employed in many industrial processes. In this work, we aimed to improve the thermostability of the serine protease PB92 from Bacillus alcalophilus to meet the high-temperature requirements of biotechnological treatments. Eight
Huabiao Miao +4 more
doaj +1 more source
A thermostable glucoamylase from a thermophilic Bacillus sp.: characterization and thermostability
Journal of Industrial Microbiology & Biotechnology, 2004 A thermostable glucoamylase (GA) showed optimum activity at 70 degrees C and pH 5.0. It was highly stable at pH 7.0. The half-life of the enzyme at pH 7.0 was 13, 8, and 3 h 40 min at 60, 65, and 70 degrees C respectively. The residual activity of the enzyme sample incubated at 5 psi (110 degrees C) for 30 min was about 32% of the control set ...
Rupinder K, Gill, Jagdeep, Kaur
openaire +2 more sources
Advanced Robotics Research, EarlyView.Amphiphilic chloroazobenzenes, bearing different amino acids as end groups alanine, phenylalanine, and valine, exhibit excellent photoisomerizations. Varying structures of three amino acid groups participate in supramolecular formations and therefore photoinduced morphological transformations via adjusting intermolecular interactions. These interaction
Shuangshuang Meng +3 more
wiley +1 more source
Understanding Thermostability Factors of Barley Limit Dextrinase by Molecular Dynamics Simulations
Frontiers in Molecular Biosciences, 2020 Limit dextrinase (LD) is the only endogenous starch-debranching enzyme in barley (Hordeum vulgare, Hv), which is the key factor affecting the production of a high degree of fermentation.
Juan Du +7 more
doaj +1 more source
Advanced Science, EarlyView.This study presents the de novo design and directed evolution of a mirror‐image D‐protein inhibitor targeting human interleukin‐4 (IL‐4). The engineered molecule exhibits nanomolar binding affinity for IL‐4 and effectively inhibits IL‐4–mediated signaling.
Liqing Xu +7 more
wiley +1 more source
Stereoselective Biotransformation: Transfer of Learning to Advance Drug Metabolism and Biocatalysis
Angewandte Chemie, EarlyView.Understanding stereoselective biotransformations has implications for predicting drug disposition and response and may also inspire novel biocatalytic and biomimetic strategies to address challenges in metabolite and API synthesis. ABSTRACT Chirality is an important determinant of drug action, as enantiomers can exhibit markedly different ...
Grace A. Okunlola, Godwin A. Aleku
wiley +2 more sources
Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions
Advanced Science, EarlyView.This review maps the fast‐evolving landscape of plant genetic engineering, linking enabling platforms with trait‐focused applications in architecture optimization, stress resilience, yield improvement, and quality enhancement. It highlights how genome editing, transgenic strategies, and emerging multi‐gene approaches reshape breeding pipelines, while ...
Peilin Wang +4 more
wiley +1 more source
Cryo-EM structure of a thermostable bacterial nanocompartment
IUCrJ, 2021 Protein nanocompartments are widespread in bacteria and archaea, but their functions are not yet well understood. Here, the cryo-EM structure of a nanocompartment from the thermophilic bacterium Thermotoga maritima is reported at 2.0 Å resolution.
Timothy Wiryaman, Navtej Toor
doaj +1 more source
Advanced Science, EarlyView.An mRNA‐liposome vaccine via dissolving microneedles (ML‐DMN) enables efficient skin‐targeted delivery while preserving mRNA integrity and allowing sustained antigen expression. Unlike intramuscular (IM) injection, ML‐DMN engages skin‐resident immune cells, activates antigen‐presenting cells (APCs), and induces strong T cell and cytokine responses at ...
Jeehye Nam +9 more
wiley +1 more source